2D6Y

Crystal Structure of transcriptional factor SCO4008 from Streptomyces coelicolor A3(2)

Summary for 2D6Y

• Entry DOI: 10.2210/pdb2d6y/pdb
• Related: 1PB6 1Z77 2TCT
• Descriptor: putative tetR family regulatory protein, L(+)-TARTARIC ACID (3 entities in total)
• Functional Keywords: helix-turn-helix, gene regulation
• Biological source: Streptomyces coelicolor
• Total number of polymer chains: 2
• Total formula weight: 46006.82

Authors

Hayashi, T.,Tanaka, Y.,Sakai, N.,Yao, M.,Tamura, T.,Tanaka, I. (deposition date: 2005-11-15, release date: 2006-10-31, Last modification date: 2024-11-06)

Primary citation

Hayashi, T.,Tanaka, Y.,Sakai, N.,Okada, U.,Yao, M.,Watanabe, N.,Tamura, T.,Tanaka, I.
SCO4008, a Putative TetR Transcriptional Repressor from Streptomyces coelicolor A3(2), Regulates Transcription of sco4007 by Multidrug Recognition.
J.Mol.Biol., 425:3289-3300, 2013

PubMed Abstract: SCO4008 from Streptomyces coelicolor A3(2) is a member of the TetR family. However, its precise function is not yet clear. In this study, the crystal structure of SCO4008 was determined at a resolution of 2.3Å, and its DNA-binding properties were analyzed. Crystal structure analysis showed that SCO4008 forms an Ω-shaped homodimer in which the monomer is composed of an N-terminal DNA-binding domain containing a helix-turn-helix and a C-terminal dimerization and regulatory domain possessing a ligand-binding cavity. The genomic systematic evolution of ligands by exponential enrichment and electrophoretic mobility shift assay revealed that four SCO4008 dimers bind to the two operator regions located between sco4008 and sco4007, a secondary transporter belonging to the major facilitator superfamily. Ligand screening analysis showed that SCO4008 recognizes a wide range of structurally dissimilar cationic and hydrophobic compounds. These results suggested that SCO4008 is a transcriptional repressor of sco4007 responsible for the multidrug resistance system in S. coelicolor A3(2).

PubMed: 23831227
DOI: 10.1016/j.jmb.2013.06.013

Experimental method

X-RAY DIFFRACTION (2.3 Å)