2D5R

Crystal Structure of a Tob-hCaf1 Complex

2D5R の概要

• エントリーDOI: 10.2210/pdb2d5r/pdb
• 分子名称: CCR4-NOT transcription complex subunit 7, Tob1 protein (3 entities in total)
• 機能のキーワード: poly(a) deadenylase, antiproliferative protein, transcription
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: Q9UIV1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 42930.09

構造登録者

Horiuchi, M.,Suzuki, N.N.,Muroya, N.,Takahasi, K.,Nishida, M.,Yoshida, Y.,Ikematsu, N.,Nakamura, T.,Kawamura-Tsuzuku, J.,Yamamoto, T.,Inagaki, F. (登録日: 2005-11-04, 公開日: 2006-12-12, 最終更新日: 2024-03-13)

主引用文献

Horiuchi, M.,Takeuchi, K.,Noda, N.,Muroya, N.,Suzuki, T.,Nakamura, T.,Kawamura-Tsuzuku, J.,Takahasi, K.,Yamamoto, T.,Inagaki, F.
Structural basis for the antiproliferative activity of the Tob-hCaf1 complex.
J.Biol.Chem., 284:13244-13255, 2009

PubMed Abstract: The Tob/BTG family is a group of antiproliferative proteins containing two highly homologous regions, Box A and Box B. These proteins all associate with CCR4-associated factor 1 (Caf1), which belongs to the ribonuclease D (RNase D) family of deadenylases and is a component of the CCR4-Not deadenylase complex. Here we determined the crystal structure of the complex of the N-terminal region of Tob and human Caf1 (hCaf1). Tob exhibited a novel fold, whereas hCaf1 most closely resembled the catalytic domain of yeast Pop2 and human poly(A)-specific ribonuclease. Interestingly, the association of hCaf1 was mediated by both Box A and Box B of Tob. Cell growth assays using both wild-type and mutant proteins revealed that deadenylase activity of Caf1 is not critical but complex formation is crucial to cell growth inhibition. Caf1 tethers Tob to the CCR4-Not deadenylase complex, and thereby Tob gathers several factors at its C-terminal region, such as poly(A)-binding proteins, to exert antiproliferative activity.

PubMed: 19276069
DOI: 10.1074/jbc.M809250200

実験手法

X-RAY DIFFRACTION (2.5 Å)