2D4Z

Crystal structure of the cytoplasmic domain of the chloride channel ClC-0

2D4Z の概要

• エントリーDOI: 10.2210/pdb2d4z/pdb
• 分子名称: Chloride channel protein (1 entity in total)
• 機能のキーワード: clc chloride channel cytoplasmic domain, cbs domains, ion channel regulatory subunit, transport protein
• 由来する生物種: Torpedo marmorata (marbled electric ray)
• 細胞内の位置: Membrane; Multi-pass membrane protein: P21564
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 55573.67

構造登録者

Dutzler, R.,Meyer, S. (登録日: 2005-10-26, 公開日: 2006-02-14, 最終更新日: 2024-03-13)

主引用文献

Meyer, S.,Dutzler, R.
Crystal structure of the cytoplasmic domain of the chloride channel ClC-0.
Structure, 14:299-307, 2006

PubMed Abstract: Ion channels are frequently organized in a modular fashion and consist of a membrane-embedded pore domain and a soluble regulatory domain. A similar organization is found for the ClC family of Cl- channels and transporters. Here, we describe the crystal structure of the cytoplasmic domain of ClC-0, the voltage-dependent Cl- channel from T. marmorata. The structure contains a folded core of two tightly interacting cystathionine beta-synthetase (CBS) subdomains. The two subdomains are connected by a 96 residue mobile linker that is disordered in the crystals. As revealed by analytical ultracentrifugation, the domains form dimers, thereby most likely extending the 2-fold symmetry of the transmembrane pore. The structure provides insight into the organization of the cytoplasmic domains within the ClC family and establishes a framework for guiding future investigations on regulatory mechanisms.

PubMed: 16472749
DOI: 10.1016/j.str.2005.10.008

実験手法

X-RAY DIFFRACTION (3.1 Å)