2D3O

Structure of Ribosome Binding Domain of the Trigger Factor on the 50S ribosomal subunit from D. radiodurans

Summary for 2D3O

• Entry DOI: 10.2210/pdb2d3o/pdb
• Descriptor: 23S RIBOSOMAL RNA, 50S RIBOSOMAL PROTEIN L23, 50S RIBOSOMAL PROTEIN L24, ... (5 entities in total)
• Functional Keywords: ribosome, trigger factor, nascent chain, 50s, protein folding, srp
• Biological source: Deinococcus radiodurans; More
• Total number of polymer chains: 5
• Total formula weight: 976595.66

Authors

Schluenzen, F.,Wilson, D.N.,Hansen, H.A.,Tian, P.,Harms, J.M.,McInnes, S.J.,Albrecht, R.,Buerger, J.,Wilbanks, S.M.,Fucini, P. (deposition date: 2005-09-30, release date: 2005-12-06, Last modification date: 2024-03-13)

Primary citation

Schlunzen, F.,Wilson, D.N.,Tian, P.,Harms, J.M.,McInnes, S.J.,Hansen, H.A.,Albrecht, R.,Buerger, J.,Wilbanks, S.M.,Fucini, P.
The Binding Mode of the Trigger Factor on the Ribosome: Implications for Protein Folding and SRP Interaction
Structure, 13:1685-1694, 2005

PubMed Abstract: This study presents the X-ray structure of the N-terminal binding domain of the D. radiodurans trigger factor (TF) in complex with the D. radiodurans large ribosomal subunit. At 3.35 A, a complete description of the interactions with ribosomal proteins L23, L29, and 23S rRNA are disclosed, many of which differ from those found previously for a heterologous bacterial-archaeal TF-ribosome complex. The beta hairpin loop of eubacterial L24, which is shorter in archaeal ribosomes, contacts the TF and severely diminishes the molecular cradle proposed to exist between the TF and ribosome. Bound to the ribosome, TF exposes a hydrophobic crevice large enough to accommodate the nascent polypeptide chain. Superimposition of the full-length TF and the signal-recognition particle (SRP) onto the complex shows that simultaneous cohabitation is possible, in agreement with biochemical data, and suggests a model for the interplay of TF, SRP, and the nascent chain during translation.

PubMed: 16271892
DOI: 10.1016/j.str.2005.08.007

Experimental method

X-RAY DIFFRACTION (3.35 Å)