2D3D

crystal structure of the RNA binding SAM domain of saccharomyces cerevisiae Vts1

2D3D の概要

• エントリーDOI: 10.2210/pdb2d3d/pdb
• 分子名称: Vts1 protein, CALCIUM ION (3 entities in total)
• 機能のキーワード: rna binding, sam domain, sre hairpin binding, rna binding protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10119.86

構造登録者

Aviv, T.,Amborski, A.N.,Zhao, X.S.,Kwan, J.J.,Johnson, P.E.,Sicheri, F.,Donaldson, L.W. (登録日: 2005-09-27, 公開日: 2006-02-14, 最終更新日: 2024-03-13)

主引用文献

Aviv, T.,Amborski, A.N.,Zhao, X.S.,Kwan, J.J.,Johnson, P.E.,Sicheri, F.,Donaldson, L.W.
The NMR and X-ray Structures of the Saccharomyces cerevisiae Vts1 SAM Domain Define a Surface for the Recognition of RNA Hairpins
J.Mol.Biol., 356:274-279, 2006

PubMed Abstract: The SAM domain of the Saccharomyces cerevisiae post-transcriptional regulator Vts1 has a high affinity towards RNA hairpins containing a CUGGC pentaloop. We present the 1.6 Angstroms X-ray crystal structure of the Vts1 SAM domain in its unliganded state, and the NMR solution structure of this domain in its RNA-bound state. Both structures reveal a canonical five helix SAM domain flanked by additional secondary structural elements at the N and C termini. The two structures are essentially identical, implying that no major structural rearrangements occur upon RNA binding. Amide chemical shift changes map the RNA-binding site to a shallow, basic patch at the junction of helix alpha5 and the loop connecting helices alpha1 and alpha2.

PubMed: 16375924
DOI: 10.1016/j.jmb.2005.11.066

実験手法

X-RAY DIFFRACTION (1.6 Å)