2D2S

Crystal Structure of the Exo84p C-terminal Domains

Summary for 2D2S

• Entry DOI: 10.2210/pdb2d2s/pdb
• Related: 2B1E
• Descriptor: Exocyst complex component EXO84 (1 entity in total)
• Functional Keywords: tethering complex, exocyst, exo84p, endocytosis-exocytosis complex, endocytosis/exocytosis
• Biological source: Saccharomyces cerevisiae (baker's yeast)
• Cellular location: Cytoplasmic vesicle, secretory vesicle: P38261
• Total number of polymer chains: 1
• Total formula weight: 26836.61

Authors

Dong, G.,Hutagalung, A.H.,Fu, C.,Novick, P.,Reinisch, K.M. (deposition date: 2005-09-16, release date: 2005-11-01, Last modification date: 2024-03-13)

Primary citation

Dong, G.,Hutagalung, A.H.,Fu, C.,Novick, P.,Reinisch, K.M.
The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif
Nat.Struct.Mol.Biol., 12:1094-1100, 2005

PubMed Abstract: The exocyst is a large complex that is required for tethering vesicles at the final stages of the exocytic pathway in all eukaryotes. Here we present the structures of the Exo70p subunit of this complex and of the C-terminal domains of Exo84p, at 2.0-A and 2.85-A resolution, respectively. Exo70p forms a 160-A-long rod with a novel fold composed of contiguous alpha-helical bundles. The Exo84p C terminus also forms a long rod (80 A), which unexpectedly has the same fold as the Exo70p N terminus. Our structural results and our experimental observations concerning the interaction between Exo70p and other exocyst subunits or Rho3p GTPase are consistent with an architecture wherein exocyst subunits are composed of mostly helical modules strung together into long rods.

PubMed: 16249794
DOI: 10.1038/nsmb1017

Experimental method

X-RAY DIFFRACTION (2.85 Å)