2D1V

Crystal structure of DNA-binding domain of Bacillus subtilis YycF

Summary for 2D1V

• Entry DOI: 10.2210/pdb2d1v/pdb
• Descriptor: Transcriptional regulatory protein yycF (2 entities in total)
• Functional Keywords: helix-turn-helix motif, dna-binding domain, two-component system, response regulator, transcription
• Biological source: Bacillus subtilis
• Cellular location: Cytoplasm : P37478
• Total number of polymer chains: 1
• Total formula weight: 12811.34

Authors

Okajima, T.,Okada, A.,Watanabe, T.,Yamamoto, K.,Tanizawa, K.,Utsumi, R. (deposition date: 2005-09-01, release date: 2006-09-12, Last modification date: 2024-03-13)

Primary citation

Okajima, T.,Doi, A.,Okada, A.,Gotoh, Y.,Tanizawa, K.,Utsumi, R.
Response regulator YycF essential for bacterial growth: X-ray crystal structure of the DNA-binding domain and its PhoB-like DNA recognition motif
Febs Lett., 582:3434-3438, 2008

PubMed Abstract: A response regulator YycF and its cognate sensor kinase YycG constitute the two-component signal transduction system essential for growth of Gram-positive bacteria with a low GC content. We have determined the X-ray crystal structure of the effector domain of Bacillus subtilis YycF involved in DNA binding. The structure, containing a winged helix-turn-helix motif, was found to be very similar to that of the response regulator PhoB from Escherichia coli. Specific binding of YycF to the PhoB-regulated alkaline phosphatase promoter was also demonstrated.

PubMed: 18789936
DOI: 10.1016/j.febslet.2008.09.007

Experimental method

X-RAY DIFFRACTION (2.4 Å)