2D1V

Crystal structure of DNA-binding domain of Bacillus subtilis YycF

2D1V の概要

• エントリーDOI: 10.2210/pdb2d1v/pdb
• 分子名称: Transcriptional regulatory protein yycF (2 entities in total)
• 機能のキーワード: helix-turn-helix motif, dna-binding domain, two-component system, response regulator, transcription
• 由来する生物種: Bacillus subtilis
• 細胞内の位置: Cytoplasm : P37478
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12811.34

構造登録者

Okajima, T.,Okada, A.,Watanabe, T.,Yamamoto, K.,Tanizawa, K.,Utsumi, R. (登録日: 2005-09-01, 公開日: 2006-09-12, 最終更新日: 2024-03-13)

主引用文献

Okajima, T.,Doi, A.,Okada, A.,Gotoh, Y.,Tanizawa, K.,Utsumi, R.
Response regulator YycF essential for bacterial growth: X-ray crystal structure of the DNA-binding domain and its PhoB-like DNA recognition motif
Febs Lett., 582:3434-3438, 2008

PubMed Abstract: A response regulator YycF and its cognate sensor kinase YycG constitute the two-component signal transduction system essential for growth of Gram-positive bacteria with a low GC content. We have determined the X-ray crystal structure of the effector domain of Bacillus subtilis YycF involved in DNA binding. The structure, containing a winged helix-turn-helix motif, was found to be very similar to that of the response regulator PhoB from Escherichia coli. Specific binding of YycF to the PhoB-regulated alkaline phosphatase promoter was also demonstrated.

PubMed: 18789936
DOI: 10.1016/j.febslet.2008.09.007

実験手法

X-RAY DIFFRACTION (2.4 Å)