2CT8

Crystal structure of Aquifex aeolicus methionyl-tRNA synthetase complexed with tRNA(Met) and methionyl-adenylate anologue

Summary for 2CT8

Entry DOI	10.2210/pdb2ct8/pdb
Related	2CSX
Descriptor	RNA (74-MER), Methionyl-tRNA synthetase, 5'-O-[(L-METHIONYL)-SULPHAMOYL]ADENOSINE, ... (4 entities in total)
Functional Keywords	ligase, riken structural genomics/proteomics initiative, rsgi, structural genomics, ligase-rna complex, ligase/rna
Biological source	Aquifex aeolicus
Cellular location	Cytoplasm: O67298
Total number of polymer chains	4
Total formula weight	166754.88
Authors	Nakanishi, K.,Ogiso, Y.,Nakama, T.,Fukai, S.,Nureki, O.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2005-05-23, release date: 2005-09-20, Last modification date: 2024-03-13)
Primary citation	Nakanishi, K.,Ogiso, Y.,Nakama, T.,Fukai, S.,Nureki, O. Structural basis for anticodon recognition by methionyl-tRNA synthetase. Nat.Struct.Mol.Biol., 12:931-932, 2005 Cited by PubMed Abstract: In the 2.7-A resolution crystal structure of methionyl-tRNA synthetase (MetRS) in complex with tRNA(Met) and a methionyl-adenylate analog, the tRNA anticodon loop is distorted to form a triple-base stack comprising C34, A35 and A38. A tryptophan residue stacks on C34 to extend the triple-base stack. In addition, C34 forms Watson-Crick-type hydrogen bonds with Arg357. This structure resolves the longstanding question of how MetRS specifically recognizes tRNA(Met). PubMed: 16155581 DOI: 10.1038/nsmb988 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.7 Å)

Structure validation