2CSE

Features of Reovirus Outer-Capsid Protein mu1 Revealed by Electron and Image Reconstruction of the virion at 7.0-A Resolution

2CSE の概要

• エントリーDOI: 10.2210/pdb2cse/pdb
• 分子名称: major outer-capsid protein mu1, major capsid surface protein sigma-3, guanylyltransferase, ... (6 entities in total)
• 機能のキーワード: cryoem, image processing, reovirus, membrane penetration protein, icosahedral virus, virus
• 由来する生物種: Mammalian orthoreovirus 1; 詳細
• タンパク質・核酸の鎖数: 27
• 化学式量合計: 1887925.71

構造登録者

Zhang, X.,Ji, Y.,Zhang, L.,Harrison, S.C.,Marinescu, D.C.,Nibert, M.L.,Baker, T.S. (登録日: 2005-05-21, 公開日: 2005-10-18, 最終更新日: 2024-02-14)

主引用文献

Zhang, X.,Ji, Y.,Zhang, L.,Harrison, S.C.,Marinescu, D.C.,Nibert, M.L.,Baker, T.S.
Features of reovirus outer capsid protein mu1 revealed by electron cryomicroscopy and image reconstruction of the virion at 7.0 Angstrom resolution.
Structure, 13:1545-1557, 2005

PubMed Abstract: Reovirus is a useful model for addressing the molecular basis of membrane penetration by one of the larger nonenveloped animal viruses. We now report the structure of the reovirus virion at approximately 7.0 A resolution as obtained by electron cryomicroscopy and three-dimensional image reconstruction. Several features of the myristoylated outer capsid protein mu1, not seen in a previous X-ray crystal structure of the mu1-sigma3 heterohexamer, are evident in the virion. These features appear to be important for stabilizing the outer capsid, regulating the conformational changes in mu1 that accompany perforation of target membranes, and contributing directly to membrane penetration during cell entry.

PubMed: 16216585
DOI: 10.1016/j.str.2005.07.012

実験手法

ELECTRON MICROSCOPY (7 Å)