2CS7

1.2 A Crystal structure of the S. pneumoniae PhtA histidine triad domain a novel zinc binding fold

2CS7 の概要

• エントリーDOI: 10.2210/pdb2cs7/pdb
• 分子名称: pneumococcal histidine triad A protein, ZINC ION (3 entities in total)
• 機能のキーワード: phta, pneumococcal histidine triad protein, s.pneumoniae, structural genomics, unknown function
• 由来する生物種: Streptococcus pneumoniae
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 18272.45

構造登録者

Riboldi-Tunnicliffe, A.,Isaacs, N.W.,Mitchell, T.J. (登録日: 2005-05-20, 公開日: 2006-02-14, 最終更新日: 2024-02-14)

主引用文献

Riboldi-Tunnicliffe, A.,Isaacs, N.W.,Mitchell, T.J.
1.2 Angstroms crystal structure of the S. pneumoniae PhtA histidine triad domain a novel zinc binding fold.
Febs Lett., 579:5353-5360, 2005

PubMed Abstract: The recently described pneumococcal histidine triad protein family has been shown to be highly conserved within the pneumococcus. As part of our structural genomics effort on proteins from Streptococcus pneumoniae, we have expressed, crystallised and solved the structure of PhtA-166-220 at 1.2 Angstroms using remote SAD with zinc. The structure of PhtA-166-220 shows no similarity to any protein structure. The overall fold contains 3beta-strands and a single short alpha-helix. The structure appears to contain a novel zinc binding motif. The remaining 4 histidine triad repeats from PhtA have been modelled based on the crystal structure of the PhtA histidine triad repeat 2. From this modelling work, we speculate that only three of the five histidine triad repeats contain the residues in the correct geometry to allow the binding of a zinc ion.

PubMed: 16194532
DOI: 10.1016/j.febslet.2005.08.066

実験手法

X-RAY DIFFRACTION (1.2 Å)