2CPU

SUBSITE MAPPING OF THE ACTIVE SITE OF HUMAN PANCREATIC ALPHA-AMYLASE USING SUBSTRATES, THE PHARMACOLOGICAL INHIBITOR ACARBOSE, AND AN ACTIVE SITE VARIANT

2CPU の概要

• エントリーDOI: 10.2210/pdb2cpu/pdb
• 分子名称: ALPHA-AMYLASE, CALCIUM ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: amylase, mutagenesis, diabetes, catalysis, pancreatic, enzyme, human, hydrolase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 56005.85

構造登録者

Brayer, G.D.,Sidhu, G.,Maurus, R.,Rydberg, E.H.,Braun, C.,Wang, Y.,Nguyen, N.T.,Overall, C.M.,Withers, S.G. (登録日: 1999-06-08, 公開日: 2001-06-30, 最終更新日: 2023-12-27)

• 主引用文献: Brayer, G.D.,Sidhu, G.,Maurus, R.,Rydberg, E.H.,Braun, C.,Wang, Y.,Nguyen, N.T.,Overall, C.M.,Withers, S.G.; Subsite mapping of the human pancreatic alpha-amylase active site through structural, kinetic, and mutagenesis techniques.; Biochemistry, 39:4778-4791, 2000; PubMed: 10769135; DOI: 10.1021/bi9921182

実験手法

X-RAY DIFFRACTION (2 Å)