2COV

Crystal structure of CBM31 from beta-1,3-xylanase

2COV の概要

• エントリーDOI: 10.2210/pdb2cov/pdb
• 分子名称: beta-1,3-xylanase (2 entities in total)
• 機能のキーワード: carbohydrate-binding module, family 31 cbm, beta-1, 3-xylanase, sugar binding protein
• 由来する生物種: Alcaligenes sp.
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 72931.78

構造登録者

Hashimoto, H.,Tamai, Y.,Okazaki, F.,Tamaru, Y.,Shimizu, T.,Araki, T.,Sato, M. (登録日: 2005-05-18, 公開日: 2005-09-13, 最終更新日: 2024-11-20)

主引用文献

Hashimoto, H.,Tamai, Y.,Okazaki, F.,Tamaru, Y.,Shimizu, T.,Araki, T.,Sato, M.
The first crystal structure of a family 31 carbohydrate-binding module with affinity to beta-1,3-xylan
Febs Lett., 579:4324-4328, 2005

PubMed Abstract: Here, we present the crystal structure of the family 31 carbohydrate-binding module (CBM) of beta-1,3-xylanase from Alcaligenes sp. strain XY-234 (AlcCBM31) determined at a resolution of 1.25A. The AlcCBM31 shows affinity with only beta-1,3-xylan. The AlcCBM31 molecule makes a beta-sandwich structure composed of eight beta-strands with a typical immunoglobulin fold and contains two intra-molecular disulfide bonds. The folding topology of AlcCBM31 differs from that of the large majority of other CBMs, in which eight beta-strands comprise a beta-sandwich structure with a typical jelly-roll fold. AlcCBM31 shows structural similarity with CBM structures of family 34 and family 9, which also adopt structures based on immunoglobulin folds.

PubMed: 16061225
DOI: 10.1016/j.febslet.2005.06.062

実験手法

X-RAY DIFFRACTION (1.25 Å)