2CN1

Crystal structure of Human Cytosolic 5'-Nucleotidase III (NT5C3)

Summary for 2CN1

• Entry DOI: 10.2210/pdb2cn1/pdb
• Descriptor: CYTOSOLIC 5'-NUCLEOTIDASE III (2 entities in total)
• Functional Keywords: nucleotide-binding, umph1, 5-prime, hydrolase, nucleotidase
• Biological source: HOMO SAPIENS
• Cellular location: Cytoplasm (Potential). Isoform 2: Endoplasmic reticulum: Q9H0P0
• Total number of polymer chains: 1
• Total formula weight: 33199.02

Authors

Wallden, K.,Stenmark, P.,Arrowsmith, C.,Berglund, H.,Collins, R.,Edwards, A.,Ehn, M.,Flodin, S.,Flores, A.,Graslund, S.,Hammarstrom, M.,Hallberg, B.M.,Holmberg Schiavone, L.,Hogbom, M.,Kotenyova, T.,Magnusdottir, A.,Nilsson-Ehle, P.,Nyman, T.,Ogg, D.,Persson, C.,Sagemark, J.,Sundstrom, M.,Thorsell, A.G.,Uppenberg, J.,Van Den Berg, S.,Weigelt, J.,Welin, M.,Nordlund, P. (deposition date: 2006-05-17, release date: 2006-06-12, Last modification date: 2023-12-13)

Primary citation

Wallden, K.,Stenmark, P.,Nyman, T.,Flodin, S.,Graslund, S.,Loppnau, P.,Bianchi, V.,Nordlund, P.
Crystal Structure of Human Cytosolic 5'-Nucleotidase II: Insights Into Allosteric Regulation and Substrate Recognition.
J.Biol.Chem., 282:17828-, 2007

PubMed Abstract: Cytosolic 5'-nucleotidase II catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates and regulates the IMP and GMP pools within the cell. It possesses phosphotransferase activity and thereby also catalyzes the reverse reaction. Both reactions are allosterically activated by adenine-based nucleotides and 2,3-bisphosphoglycerate. We have solved structures of cytosolic 5'-nucleotidase II as native protein (2.2 Angstrom) and in complex with adenosine (1.5 Angstrom) and beryllium trifluoride (2.15 Angstrom) The tetrameric enzyme is structurally similar to enzymes of the haloacid dehalogenase (HAD) superfamily, including mitochondrial 5'(3')-deoxyribonucleotidase and cytosolic 5'-nucleotidase III but possesses additional regulatory regions that contain two allosteric effector sites. At effector site 1 located near a subunit interface we modeled diadenosine tetraphosphate with one adenosine moiety in each subunit. This efficiently glues the tetramer subunits together in pairs. The model shows why diadenosine tetraphosphate but not diadenosine triphosphate activates the enzyme and supports a role for cN-II during apoptosis when the level of diadenosine tetraphosphate increases. We have also modeled 2,3-bisphosphoglycerate in effector site 1 using one phosphate site from each subunit. By comparing the structure of cytosolic 5'-nucleotidase II with that of mitochondrial 5'(3')-deoxyribonucleotidase in complex with dGMP, we identified residues involved in substrate recognition.

PubMed: 17405878
DOI: 10.1074/JBC.M700917200

Experimental method

X-RAY DIFFRACTION (2.67 Å)