2CKY

Structure of the Arabidopsis thaliana thiamine pyrophosphate riboswitch with its regulatory ligand

Summary for 2CKY

• Entry DOI: 10.2210/pdb2cky/pdb
• Descriptor: NUCLEIC ACID, MAGNESIUM ION, OSMIUM ION, ... (4 entities in total)
• Functional Keywords: nucleic acid
• Biological source: ARABIDOPSIS THALIANA (MOUSE-EAR CRESS)
• Total number of polymer chains: 2
• Total formula weight: 53294.11

Authors

Thore, S.,Leibundgut, M.,Ban, N. (deposition date: 2006-04-24, release date: 2006-05-08, Last modification date: 2024-05-08)

Primary citation

Thore, S.,Leibundgut, M.,Ban, N.
Structure of the Eukaryotic Thiamine Pyrophosphate Riboswitch with its Regulatory Ligand.
Science, 312:1208-, 2006

PubMed Abstract: Riboswitches are untranslated regions of messenger RNA, which adopt alternate structures depending on the binding of specific metabolites. Such conformational switching regulates the expression of proteins involved in the biosynthesis of riboswitch substrates. Here, we present the 2.9 angstrom-resolution crystal structure of the eukaryotic Arabidopsis thaliana thiamine pyrophosphate (TPP)-specific riboswitch in complex with its natural ligand. The riboswitch specifically recognizes the TPP via conserved residues located within two highly distorted parallel "sensor" helices. The structure provides the basis for understanding the reorganization of the riboswitch fold upon TPP binding and explains the mechanism of resistance to the antibiotic pyrithiamine.

PubMed: 16675665
DOI: 10.1126/SCIENCE.1128451

Experimental method

X-RAY DIFFRACTION (2.9 Å)