2CKY

Structure of the Arabidopsis thaliana thiamine pyrophosphate riboswitch with its regulatory ligand

2CKY の概要

• エントリーDOI: 10.2210/pdb2cky/pdb
• 分子名称: NUCLEIC ACID, MAGNESIUM ION, OSMIUM ION, ... (4 entities in total)
• 機能のキーワード: nucleic acid
• 由来する生物種: ARABIDOPSIS THALIANA (MOUSE-EAR CRESS)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 53294.11

構造登録者

Thore, S.,Leibundgut, M.,Ban, N. (登録日: 2006-04-24, 公開日: 2006-05-08, 最終更新日: 2024-05-08)

主引用文献

Thore, S.,Leibundgut, M.,Ban, N.
Structure of the Eukaryotic Thiamine Pyrophosphate Riboswitch with its Regulatory Ligand.
Science, 312:1208-, 2006

PubMed Abstract: Riboswitches are untranslated regions of messenger RNA, which adopt alternate structures depending on the binding of specific metabolites. Such conformational switching regulates the expression of proteins involved in the biosynthesis of riboswitch substrates. Here, we present the 2.9 angstrom-resolution crystal structure of the eukaryotic Arabidopsis thaliana thiamine pyrophosphate (TPP)-specific riboswitch in complex with its natural ligand. The riboswitch specifically recognizes the TPP via conserved residues located within two highly distorted parallel "sensor" helices. The structure provides the basis for understanding the reorganization of the riboswitch fold upon TPP binding and explains the mechanism of resistance to the antibiotic pyrithiamine.

PubMed: 16675665
DOI: 10.1126/SCIENCE.1128451

実験手法

X-RAY DIFFRACTION (2.9 Å)