2CDV

REFINED STRUCTURE OF CYTOCHROME C3 AT 1.8 ANGSTROMS RESOLUTION

「1CDV」から置き換えられました

2CDV の概要

• エントリーDOI: 10.2210/pdb2cdv/pdb
• NMR情報: BMRB: 5333
• 分子名称: CYTOCHROME C3, HEME C (3 entities in total)
• 機能のキーワード: heme protein of electron transport
• 由来する生物種: Desulfovibrio vulgaris
• 細胞内の位置: Periplasm: P00132
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14031.31

構造登録者

Higuchi, Y.,Kusunoki, M.,Matsuura, Y.,Yasuoka, N.,Kakudo, M. (登録日: 1983-11-15, 公開日: 1984-02-02, 最終更新日: 2024-10-23)

主引用文献

Higuchi, Y.,Kusunoki, M.,Matsuura, Y.,Yasuoka, N.,Kakudo, M.
Refined structure of cytochrome c3 at 1.8 A resolution
J.Mol.Biol., 172:109-139, 1984

PubMed Abstract: The structure of cytochrome c3 from the sulfate-reducing bacterium Desulfovibrio vulgaris Miyazaki has been successfully refined at 1.8 A resolution. The crystallographic R factor is 0.176 for 9907 significant reflections. The isotropic temperature factors of individual atoms were refined and a total of 47 water molecules located on the difference map were incorporated in the refinement. The four heme groups are closely packed, with adjacent pairs of heme planes being nearly perpendicular to each other. The fifth and the sixth ligands of the heme iron atoms are histidine residues with N epsilon 2-Fe distances ranging from 1.88 A to 2.12 A. The histidine co-ordination to the heme iron is different for each heme group. The heme groups are all highly exposed to solvent, although the actual regions exposed differ among the hemes. The four heme groups are located in different environments, and the heme planes are deformed from planarity. The differences in the heme structures and their environments indicate that the four heme groups are non-equivalent. The chemical as well as the physical properties of cytochrome c3 should be interpreted in terms of the structural non-equivalence of the heme groups. The characteristic secondary structural non-equivalence of the heme groups. The characteristic secondary structures of the polypeptide chain of this molecule are three short alpha-helices, two short beta-strands and ten reverse turns.

PubMed: 6319712
DOI: 10.1016/0022-2836(84)90417-0

実験手法

X-RAY DIFFRACTION (1.8 Å)