2CBS

CELLULAR RETINOIC ACID BINDING PROTEIN II IN COMPLEX WITH A SYNTHETIC RETINOIC ACID (RO-13 6307)

2CBS の概要

• エントリーDOI: 10.2210/pdb2cbs/pdb
• 分子名称: PROTEIN (CRABP-II), 3-METHYL-7-(5,5,8,8-TETRAMETHYL-5,6,7,8-TETRAHYDRO-NAPHTHALEN-2-YL) -OCTA-2,4,6-TRIENOIC ACID (3 entities in total)
• 機能のキーワード: retinoic-acid transport, transport protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15920.28

構造登録者

Chaudhuri, B.,Kleywegt, G.J.,Bergfors, T.,Jones, T.A. (登録日: 1999-02-22, 公開日: 1999-12-22, 最終更新日: 2023-08-23)

主引用文献

Chaudhuri, B.N.,Kleywegt, G.J.,Broutin-L'Hermite, I.,Bergfors, T.,Senn, H.,Le Motte, P.,Partouche, O.,Jones, T.A.
Structures of cellular retinoic acid binding proteins I and II in complex with synthetic retinoids.
Acta Crystallogr.,Sect.D, 55:1850-1857, 1999

PubMed Abstract: Retinoids play important roles in diverse cellular processes including growth, cell differentiation and vision. Many natural and synthetic retinoids are used as drugs in dermatology and oncology. A large amount of data has been accumulated on the cellular activity of different synthetic retinoids. They are stabilized and transported inside the cell cytoplasm by binding and transport proteins, such as cellular retinol-binding proteins and cellular retinoic acid binding proteins (CRABPs). The structures of human CRABP II in complex with two different synthetic retinoids, Ro13-6307 and Ro12--7310 (at 2.1 and 2.0 A resolution, respectively) and of bovine CRABP I in complex with a retinobenzoic acid, Am80 (at 2.8 A resolution) are described. The binding affinities of human CRABP I and II for the retinoids studied here have been determined. All these compounds have comparable binding affinities (nanomolar range) for both CRABPs. Apart from the particular interactions of the carboxylate group of the retinoids with specific protein groups, each structure reveals characteristic interactions. Studying the atomic details of the interaction of retinoids with retinoid-binding proteins facilitates the understanding of the kinetics of retinoid trafficking inside the cytoplasm.

PubMed: 10531482
DOI: 10.1107/S0907444999011026

実験手法

X-RAY DIFFRACTION (2.1 Å)