2C9F

THE QUASI-ATOMIC MODEL OF THE ADENOVIRUS TYPE 3 PENTON DODECAHEDRON

2C9F の概要

• エントリーDOI: 10.2210/pdb2c9f/pdb
• 関連するPDBエントリー: 1X9P 1X9T 2BLD 2BVI 2C6S 2C9G
• EMDBエントリー: 1178
• 分子名称: PENTON PROTEIN, FIBER (2 entities in total)
• 機能のキーワード: virus-viral protein complex, dodecahedron, penton, fibre, virus/viral protein
• 由来する生物種: HUMAN ADENOVIRUS TYPE 2; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 302185.76

構造登録者

Fuschiotti, P.,Schoehn, G.,Fender, P.,Fabry, C.M.S.,Hewat, E.A.,Chroboczek, J.,Ruigrok, R.W.H.,Conway, J.F. (登録日: 2005-12-12, 公開日: 2006-03-02, 最終更新日: 2024-05-08)

主引用文献

Fuschiotti, P.,Schoehn, G.,Fender, P.,Fabry, C.M.S.,Hewat, E.A.,Chroboczek, J.,Ruigrok, R.W.H.,Conway, J.F.
Structure of the Dodecahedral Penton Particle from Human Adenovirus Type 3.
J.Mol.Biol., 356:510-, 2006

PubMed Abstract: The sub-viral dodecahedral particle of human adenovirus type 3, composed of the viral penton base and fiber proteins, shares an important characteristic of the entire virus: it can attach to cells and penetrate them. Structure determination of the fiberless dodecahedron by cryo-electron microscopy to 9 Angstroms resolution reveals tightly bound pentamer subunits, with only minimal interfaces between penton bases stabilizing the fragile dodecahedron. The internal cavity of the dodecahedron is approximately 80 Angstroms in diameter, and the interior surface is accessible to solvent through perforations of approximately 20 Angstroms diameter between the pentamer towers. We observe weak density beneath pentamers that we attribute to a penton base peptide including residues 38-48. The intact amino-terminal domain appears to interfere with pentamer-pentamer interactions and its absence by mutation or proteolysis is essential for dodecamer assembly. Differences between the 9 Angstroms dodecahedron structure and the adenovirus serotype 2 (Ad2) crystallographic model correlate closely with differences in sequence. The 3D structure of the dodecahedron including fibers at 16 Angstroms resolution reveals extra density on the top of the penton base that can be attributed to the fiber N terminus. The fiber itself exhibits striations that correlate with features of the atomic structure of the partial Ad2 fiber and that represent a repeat motif present in the amino acid sequence. These new observations offer important insights into particle assembly and stability, as well as the practicality of using the dodecahedron in targeted drug delivery. The structural work provides a sound basis for manipulating the properties of this particle and thereby enhancing its value for such therapeutic use.

PubMed: 16375921
DOI: 10.1016/J.JMB.2005.11.048

実験手法

ELECTRON MICROSCOPY (16.5 Å)