2C7A

STRUCTURE OF THE PROGESTERONE RECEPTOR-DNA COMPLEX

2C7A の概要

• エントリーDOI: 10.2210/pdb2c7a/pdb
• 関連するPDBエントリー: 1A28 1E3K 1SQN 1SR7 1ZUC
• 分子名称: PROGESTERONE RECEPTOR, 5'-D(*CP*CP*AP*GP*AP*AP*CP*AP*GP*TP *TP*TP*GP*TP*TP*CP*TP*G)-3', 5'-D(*CP*CP*AP*GP*AP*AP*CP*AP*AP*AP *CP*TP*GP*TP*TP*CP*TP*G)-3', ... (5 entities in total)
• 機能のキーワード: receptor/dna, progesterone receptor, dna-binding, complex, metal-binding, nuclear protein, phosphorylation, steroid-binding, transcription regulation, zinc-finger, zinc, receptor-dna complex
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Nucleus. Isoform A: Nucleus: P06401
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 28605.62

構造登録者

Roemer, S.C.,Donham, D.C.,Sherman, L.,Pon, V.H.,Edwards, D.P.,Churchill, M.E.A. (登録日: 2005-11-19, 公開日: 2006-08-30, 最終更新日: 2024-05-08)

主引用文献

Roemer, S.C.,Donham, D.C.,Sherman, L.,Pon, V.H.,Edwards, D.P.,Churchill, M.E.A.
Structure of the Progesterone Receptor-Deoxyribonucleic Acid Complex: Novel Interactions Required for Binding to Half-Site Response Elements.
Mol.Endocrinol., 20:3042-, 2006

PubMed Abstract: The DNA binding domain (DBD) of nuclear hormone receptors contains a highly conserved globular domain and a less conserved carboxyl-terminal extension (CTE). Despite previous observations that the CTEs of some classes of nuclear receptors are structured and interact with DNA outside of the hexanucleotide hormone response element (HRE), there has been no evidence for such a CTE among the steroid receptors. We have determined the structure of the progesterone receptor (PR)-DBD-CTE DNA complex at a resolution of 2.5 A, which revealed binding of the CTE to the minor groove flanking the HREs. Alanine substitutions of the interacting CTE residues reduced affinity for inverted repeat HREs separated by three nucleotides, and essentially abrogated binding to a single HRE. A highly compressed minor groove of the trinucleotide spacer and a novel dimerization interface were also observed. A PR binding site selection experiment revealed sequence preferences in the trinucleotide spacer and flanking DNA. These results, taken together, support the notion that sequences outside of the HREs influence the DNA binding affinity and specificity of steroid receptors.

PubMed: 16931575
DOI: 10.1210/ME.2005-0511

実験手法

X-RAY DIFFRACTION (2.5 Å)