2C6R

FE-SOAKED CRYSTAL STRUCTURE OF THE DPS92 FROM DEINOCOCCUS RADIODURANS

2C6R の概要

• エントリーDOI: 10.2210/pdb2c6r/pdb
• 関連するPDBエントリー: 2C2J
• 分子名称: DNA-BINDING STRESS RESPONSE PROTEIN, DPS FAMILY, FE (III) ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: dps, deinococcus, radiodurans, dna-binding protein, dna binding protein
• 由来する生物種: DEINOCOCCUS RADIODURANS
• 細胞内の位置: Cytoplasm (By similarity): Q9RZN1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23604.39

構造登録者

Cuypers, M.G.,Romao, C.V.,Mitchell, E.,Mcsweeney, S. (登録日: 2005-11-11, 公開日: 2007-02-20, 最終更新日: 2024-05-08)

主引用文献

Cuypers, M.G.,Mitchell, E.P.,Romao, C.V.,Mcsweeney, S.M.
The Crystal Structure of the Dps2 from Deinococcus Radiodurans Reveals an Unusual Pore Profile with a Non-Specific Metal Binding Site.
J.Mol.Biol., 371:787-, 2007

PubMed Abstract: The crystal structure of recombinant Dps2 (DRB0092, DNA protecting protein under starved conditions) from the Gram-positive, radiation-resistant bacterium Deinococcus radiodurans has been determined in its apo and iron loaded states. Like other members of the Dps family, the bacterial DrDps2 assembles as a spherical dodecamer with an outer shell diameter of 90 A and an interior diameter of 40 A. A total of five iron sites were located in the iron loaded structure, representing the first stages of iron biomineralisation. Each subunit contains a mononuclear iron ferroxidase centre coordinated by residues highly conserved amongst the Dps family of proteins. In the structures presented, a distinct iron site is observed 6.1 A from the ferroxidase centre with a unique ligand configuration of mono coordination by the protein and no bridging ligand to the ferroxidase centre. A non-specific metallic binding site, suspected to play a regulative role in iron uptake/release from the cage, was found in a pocket located near to the external edge of the C-terminal 3-fold channel.

PubMed: 17583727
DOI: 10.1016/J.JMB.2006.11.032

実験手法

X-RAY DIFFRACTION (2.1 Å)