2C4U

Crystal structure of the apo form of the 5'-Fluoro-5'-deoxyadenosine synthase enzyme from Streptomyces cattleya

2C4U の概要

• エントリーDOI: 10.2210/pdb2c4u/pdb
• 関連するPDBエントリー: 1RQP 1RQR 2C2W 2C4T
• 分子名称: 5'-FLUORO-5'-DEOXYADENOSINE SYNTHASE, GLYCEROL (3 entities in total)
• 機能のキーワード: transferase, fluorinase, apo, sam, fda, 5'-fluorodeoxyadanosine synthase
• 由来する生物種: STREPTOMYCES CATTLEYA
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 195421.97

構造登録者

McEwan, A.R.,Deng, H.,Robinson, D.A.,DeLaurentis, W.,McGlinchey, R.P.,O'Hagan, D.,Naismith, J.H. (登録日: 2005-10-22, 公開日: 2006-04-12, 最終更新日: 2023-12-13)

主引用文献

Cobb, S.L.,Deng, H.,McEwan, A.R.,Naismith, J.H.,O'Hagan, D.,Robinson, D.A.
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates.
Org. Biomol. Chem., 4:1458-1460, 2006

PubMed Abstract: The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C-F bond. We now report that the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C-F bond formation.

PubMed: 16604208
DOI: 10.1039/b600574h

実験手法

X-RAY DIFFRACTION (2.5 Å)