2C4I

Crystal structure of engineered avidin

2C4I の概要

• エントリーDOI: 10.2210/pdb2c4i/pdb
• 関連するPDBエントリー: 1AVD 1AVE 1IJ8 1LDO 1LDQ 1LEL 1NQN 1RAV 1VYO 2AVI 2CAM
• 分子名称: AVIDIN, BIOTIN, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: biotin, glycoprotein, signalor
• 由来する生物種: GALLUS GALLUS (CHICKEN)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30194.75

構造登録者

Hytonen, V.P.,Horha, J.,Airenne, T.T.,Niskanen, E.A.,Helttunen, K.,Johnson, M.S.,Salminen, T.A.,Kulomaa, M.S.,Nordlund, H.R. (登録日: 2005-10-19, 公開日: 2006-07-05, 最終更新日: 2024-11-06)

主引用文献

Hytonen, V.P.,Horha, J.,Airenne, T.T.,Niskanen, E.A.,Helttunen, K.,Johnson, M.S.,Salminen, T.A.,Kulomaa, M.S.,Nordlund, H.R.
Controlling Quaternary Structure Assembly: Subunit Interface Engineering and Crystal Structure of Dual Chain Avidin.
J.Mol.Biol., 359:1352-, 2006

PubMed Abstract: Dual chain avidin (dcAvd) is an engineered avidin form, in which two circularly permuted chicken avidin monomers are fused into one polypeptide chain. DcAvd can theoretically form two different pseudotetrameric quaternary assemblies because of symmetry at the monomer-monomer interfaces. Here, our aim was to control the assembly of the quaternary structure of dcAvd. We introduced the mutation I117C into one of the circularly permuted domains of dcAvd and scanned residues along the 1-3 subunit interface of the other domain. Interestingly, V115H resulted in a single, disulfide locked quaternary assembly of dcAvd, whereas I117H could not guide the oligomerisation process even though it stabilised the protein. The modified dcAvd forms were found to retain their characteristic pseudotetrameric state both at high and low pH, and were shown to bind D-biotin at levels comparable to that of wild-type chicken avidin. The crystal structure of dcAvd-biotin complex at 1.95 Angstroms resolution demonstrates the formation of the functional dcAvd pseudotetramer at the atomic level and reveals the molecular basis for its special properties. Altogether, our data facilitate further engineering of the biotechnologically valuable dcAvd scaffold and gives insights into how to guide the quaternary structure assembly of oligomeric proteins.

PubMed: 16787776
DOI: 10.1016/J.JMB.2006.04.044

実験手法

X-RAY DIFFRACTION (1.95 Å)