2C1N

Molecular basis for the recognition of phosphorylated and phosphoacetylated histone H3 by 14-3-3

2C1N の概要

• エントリーDOI: 10.2210/pdb2c1n/pdb
• 関連するPDBエントリー: 1IB1 1QJA 1QJB 2C1J
• 分子名称: 14-3-3 PROTEIN ZETA/DELTA, HISTONE H3 ACETYLPHOSPHOPEPTIDE (3 entities in total)
• 機能のキーワード: signaling protein-complex, histone h3, nucleosome, signaling protein
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 60371.17

構造登録者

Welburn, J.P.I.,Macdonald, N.,Noble, M.E.M.,Nguyen, A.,Yaffe, M.B.,Clynes, D.,Moggs, J.G.,Orphanides, G.,Thomson, S.,Edmunds, J.W.,Clayton, A.L.,Endicott, J.A.,Mahadevan, L.C. (登録日: 2005-09-16, 公開日: 2005-11-02, 最終更新日: 2024-10-23)

主引用文献

Macdonald, N.,Welburn, J.P.I.,Noble, M.E.M.,Nguyen, A.,Yaffe, M.B.,Clynes, D.,Moggs, J.G.,Orphanides, G.,Thomson, S.,Edmunds, J.W.,Clayton, A.L.,Endicott, J.A.,Mahadevan, L.C.
Molecular Basis for the Recognition of Phosphorylated and Phosphoacetylated Histone H3 by 14-3-3.
Mol.Cell, 20:199-, 2005

PubMed Abstract: Phosphorylation of histone H3 is implicated in transcriptional activation and chromosome condensation, but its immediate molecular function has remained obscure. By affinity chromatography of nuclear extracts against modified H3 tail peptides, we identified 14-3-3 isoforms as proteins that bind these tails in a strictly phosphorylation-dependent manner. Acetylation of lysines 9 and 14 does not impede 14-3-3 binding to serine 10-phosphorylated H3 tails. In vivo, 14-3-3 is inducibly recruited to c-fos and c-jun nucleosomes upon gene activation, concomitant with H3 phosphoacetylation. We have determined the structures of 14-3-3zeta complexed with serine 10-phosphorylated or phosphoacetylated H3 peptides. These reveal a distinct mode of 14-3-3/phosphopeptide binding and provide a structural understanding for the lack of effect of acetylation at lysines 9 and 14 on this interaction. 14-3-3 isoforms thus represent a class of proteins that mediate the effect of histone phosphorylation at inducible genes.

PubMed: 16246723
DOI: 10.1016/J.MOLCEL.2005.08.032

実験手法

X-RAY DIFFRACTION (2 Å)