2C1J

Molecular basis for the recognition of phosphorylated and phosphoacetylated histone H3 by 14-3-3

Summary for 2C1J

• Entry DOI: 10.2210/pdb2c1j/pdb
• Related: 1IB1 1QJA 1QJB
• Descriptor: 14-3-3 PROTEIN ZETA/DELTA, HISTONE H3 ACETYLPHOSPHOPEPTIDE (3 entities in total)
• Functional Keywords: signaling protein-complex, nucleosome, signaling protein
• Biological source: HOMO SAPIENS (HUMAN); More
• Total number of polymer chains: 4
• Total formula weight: 60453.22

Authors

Welburn, J.P.I.,Macdonald, N.,Noble, M.E.M.,Nguyen, A.,Yaffe, M.B.,Clynes, D.,Moggs, J.G.,Orphanides, G.,Thomson, S.,Edmunds, J.W.,Clayton, A.L.,Endicott, J.A.,Mahadevan, L.C. (deposition date: 2005-09-15, release date: 2005-11-02, Last modification date: 2023-12-13)

Primary citation

Macdonald, N.,Welburn, J.P.I.,Noble, M.E.M.,Nguyen, A.,Yaffe, M.B.,Clynes, D.,Moggs, J.G.,Orphanides, G.,Thomson, S.,Edmunds, J.W.,Clayton, A.L.,Endicott, J.A.,Mahadevan, L.C.
Molecular Basis for the Recognition of Phosphorylated and Phosphoacetylated Histone H3 by 14-3-3.
Mol.Cell, 20:199-, 2005

PubMed Abstract: Phosphorylation of histone H3 is implicated in transcriptional activation and chromosome condensation, but its immediate molecular function has remained obscure. By affinity chromatography of nuclear extracts against modified H3 tail peptides, we identified 14-3-3 isoforms as proteins that bind these tails in a strictly phosphorylation-dependent manner. Acetylation of lysines 9 and 14 does not impede 14-3-3 binding to serine 10-phosphorylated H3 tails. In vivo, 14-3-3 is inducibly recruited to c-fos and c-jun nucleosomes upon gene activation, concomitant with H3 phosphoacetylation. We have determined the structures of 14-3-3zeta complexed with serine 10-phosphorylated or phosphoacetylated H3 peptides. These reveal a distinct mode of 14-3-3/phosphopeptide binding and provide a structural understanding for the lack of effect of acetylation at lysines 9 and 14 on this interaction. 14-3-3 isoforms thus represent a class of proteins that mediate the effect of histone phosphorylation at inducible genes.

PubMed: 16246723
DOI: 10.1016/J.MOLCEL.2005.08.032

Experimental method

X-RAY DIFFRACTION (2.6 Å)