2C0J

Crystal structure of the bet3-trs33 heterodimer

2C0J の概要

• エントリーDOI: 10.2210/pdb2c0j/pdb
• 関連するPDBエントリー: 1SZ7
• 分子名称: TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 3, R32611_2, PALMITIC ACID, ... (4 entities in total)
• 機能のキーワード: transport, transport protein, endoplasmic reticulum, er-golgi transport, lipoprotein, palmitate
• 由来する生物種: HOMO SAPIENS; 詳細
• 細胞内の位置: Golgi apparatus, cis-Golgi network : O43617 O75865
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36164.71

構造登録者

Kim, M.-S.,Yi, M.-J.,Lee, K.-H.,Wagner, J.,Munger, C.,Kim, Y.-G.,Whiteway, M.,Cygler, M.,Oh, B.-H.,Sacher, M. (登録日: 2005-09-03, 公開日: 2006-02-07, 最終更新日: 2024-10-09)

主引用文献

Kim, M.-S.,Yi, M.-J.,Lee, K.-H.,Wagner, J.,Munger, C.,Kim, Y.-G.,Whiteway, M.,Cygler, M.,Oh, B.-H.,Sacher, M.
Biochemical and Crystallographic Studies Reveal a Specific Interaction between Trapp Subunits Trs33P and Bet3P
Traffic, 6:1183-, 2005

PubMed Abstract: Transport protein particle (TRAPP) comprises a family of two highly related multiprotein complexes, with seven common subunits, that serve to target different classes of transport vesicles to their appropriate compartments. Defining the architecture of the complexes will advance our understanding of the functional differences between these highly related molecular machines. Genetic analyses in yeast suggested a specific interaction between the TRAPP subunits Bet3p and Trs33p. A mammalian bet3-trs33 complex was crystallized, and the structure was solved to 2.2 angstroms resolution. Intriguingly, the overall fold of the bet3 and trs33 monomers was similar, although the proteins had little overall sequence identity. In vitro experiments using yeast TRAPP subunits indicated that Bet3p binding to Trs33p facilitates the interaction between Bet3p and another TRAPP subunit, Bet5p. Mutational analysis suggests that yeast Trs33p facilitates other Bet3p protein-protein interactions. Furthermore, we show that Trs33p can increase the Golgi-localized pool of a mutated Bet3 protein normally found in the cytosol. We propose that one of the roles of Trs33p is to facilitate the incorporation of the Bet3p subunit into assembling TRAPP complexes.

PubMed: 16262728
DOI: 10.1111/J.1600-0854.2005.00352.X

実験手法

X-RAY DIFFRACTION (2.2 Å)