2BZF

Structural basis for DNA bridging by barrier-to-autointegration factor (BAF)

Summary for 2BZF

• Entry DOI: 10.2210/pdb2bzf/pdb
• Related: 1CI4 1QCK 2EZX 2EZY 2EZZ
• Descriptor: BARRIER-TO-AUTOINTEGRATION FACTOR, 5'-D(*GP*TP*GP*GP*AP*GP*GP)-3', 5'-D(*CP*CP*TP*CP*CP*AP*CP)-3', ... (4 entities in total)
• Functional Keywords: dna-binding protein, retroviral integration, non-specific dna-binding, dna compaction, nuclear organization, lem family, dna binding protein
• Biological source: HOMO SAPIENS (HUMAN)
• Cellular location: Nucleus: O75531
• Total number of polymer chains: 3
• Total formula weight: 14310.41

Authors

Bradley, C.M.,Ronning, D.R.,Ghirlando, R.,Craigie, R.,Dyda, F. (deposition date: 2005-08-16, release date: 2005-09-15, Last modification date: 2023-12-13)

Primary citation

Bradley, C.M.,Ronning, D.R.,Ghirlando, R.,Craigie, R.,Dyda, F.
Structural Basis for DNA Bridging by Barrier-to-Autointegration Factor.
Nat.Struct.Mol.Biol., 12:935-, 2005

PubMed Abstract: The ability of barrier-to-autointegration factor (BAF) to bind and bridge DNA in a sequence-independent manner is crucial for its role in retroviral integration and a variety of cellular processes. To better understand this behavior, we solved the crystal structure of BAF bound to DNA. The structure reveals that BAF bridges DNA using two pairs of helix-hairpin-helix motifs located on opposite surfaces of the BAF dimer without changing its conformation.

PubMed: 16155580
DOI: 10.1038/NSMB989

Experimental method

X-RAY DIFFRACTION (2.87 Å)