2BZF

Structural basis for DNA bridging by barrier-to-autointegration factor (BAF)

2BZF の概要

• エントリーDOI: 10.2210/pdb2bzf/pdb
• 関連するPDBエントリー: 1CI4 1QCK 2EZX 2EZY 2EZZ
• 分子名称: BARRIER-TO-AUTOINTEGRATION FACTOR, 5'-D(*GP*TP*GP*GP*AP*GP*GP)-3', 5'-D(*CP*CP*TP*CP*CP*AP*CP)-3', ... (4 entities in total)
• 機能のキーワード: dna-binding protein, retroviral integration, non-specific dna-binding, dna compaction, nuclear organization, lem family, dna binding protein
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Nucleus: O75531
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 14310.41

構造登録者

Bradley, C.M.,Ronning, D.R.,Ghirlando, R.,Craigie, R.,Dyda, F. (登録日: 2005-08-16, 公開日: 2005-09-15, 最終更新日: 2023-12-13)

主引用文献

Bradley, C.M.,Ronning, D.R.,Ghirlando, R.,Craigie, R.,Dyda, F.
Structural Basis for DNA Bridging by Barrier-to-Autointegration Factor.
Nat.Struct.Mol.Biol., 12:935-, 2005

PubMed Abstract: The ability of barrier-to-autointegration factor (BAF) to bind and bridge DNA in a sequence-independent manner is crucial for its role in retroviral integration and a variety of cellular processes. To better understand this behavior, we solved the crystal structure of BAF bound to DNA. The structure reveals that BAF bridges DNA using two pairs of helix-hairpin-helix motifs located on opposite surfaces of the BAF dimer without changing its conformation.

PubMed: 16155580
DOI: 10.1038/NSMB989

実験手法

X-RAY DIFFRACTION (2.87 Å)