2BWP

5-Aminolevulinate Synthase from Rhodobacter capsulatus in complex with glycine

2BWP の概要

• エントリーDOI: 10.2210/pdb2bwp/pdb
• 関連するPDBエントリー: 2BWN 2BWO
• 分子名称: 5-AMINOLEVULINATE SYNTHASE, N-GLYCINE-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YL-METHANE], ACETIC ACID, ... (4 entities in total)
• 機能のキーワード: tetrapyrrole biosynthesis, heme biosynthesis, pyridoxal phosphate dependent, transferase, acyltransferase
• 由来する生物種: RHODOBACTER CAPSULATUS
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 176100.63

構造登録者

Astner, I.,Schulze, J.O.,Van Den Heuvel, J.J.,Jahn, D.,Schubert, W.-D.,Heinz, D.W. (登録日: 2005-07-15, 公開日: 2005-09-27, 最終更新日: 2024-05-08)

主引用文献

Astner, I.,Schulze, J.O.,Van Den Heuvel, J.J.,Jahn, D.,Schubert, W.-D.,Heinz, D.W.
Crystal Structure of 5-Aminolevulinate Synthase, the First Enzyme of Heme Biosynthesis, and its Link to Xlsa in Humans.
Embo J., 24:3166-, 2005

PubMed Abstract: 5-Aminolevulinate synthase (ALAS) is the first and rate-limiting enzyme of heme biosynthesis in humans, animals, other non-plant eukaryotes, and alpha-proteobacteria. It catalyzes the synthesis of 5-aminolevulinic acid, the first common precursor of all tetrapyrroles, from glycine and succinyl-coenzyme A (sCoA) in a pyridoxal 5'-phosphate (PLP)-dependent manner. X-linked sideroblastic anemias (XLSAs), a group of severe disorders in humans characterized by inadequate formation of heme in erythroblast mitochondria, are caused by mutations in the gene for erythroid eALAS, one of two human genes for ALAS. We present the first crystal structure of homodimeric ALAS from Rhodobacter capsulatus (ALAS(Rc)) binding its cofactor PLP. We, furthermore, present structures of ALAS(Rc) in complex with the substrates glycine or sCoA. The sequence identity of ALAS from R. capsulatus and human eALAS is 49%. XLSA-causing mutations may thus be mapped, revealing the molecular basis of XLSA in humans. Mutations are found to obstruct substrate binding, disrupt the dimer interface, or hamper the correct folding. The structure of ALAS completes the structural analysis of enzymes in heme biosynthesis.

PubMed: 16121195
DOI: 10.1038/SJ.EMBOJ.7600792

実験手法

X-RAY DIFFRACTION (2.7 Å)