2BW3

Three-dimensional structure of the Hermes DNA transposase

2BW3 の概要

• エントリーDOI: 10.2210/pdb2bw3/pdb
• 分子名称: TRANSPOSASE (3 entities in total)
• 機能のキーワード: dna recombination, transposition
• 由来する生物種: MUSCA DOMESTICA (HOUSE FLY); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 71010.54

構造登録者

Hickman, A.B.,Perez, Z.N.,Zhou, L.,Musingarimi, P.,Ghirlando, R.,Hinshaw, J.E.,Craig, N.L.,Dyda, F. (登録日: 2005-07-11, 公開日: 2005-07-28, 最終更新日: 2024-11-13)

主引用文献

Hickman, A.B.,Perez, Z.N.,Zhou, L.,Musingarimi, P.,Ghirlando, R.,Hinshaw, J.E.,Craig, N.L.,Dyda, F.
Molecular Architecture of a Eukaryotic DNA Transposase
Nat.Struct.Mol.Biol., 12:715-, 2005

PubMed Abstract: Mobile elements and their inactive remnants account for large proportions of most eukaryotic genomes, where they have had central roles in genome evolution. Over 50 years ago, McClintock reported a form of stress-induced genome instability in maize in which discrete DNA segments move between chromosomal locations. Our current mechanistic understanding of enzymes catalyzing transposition is largely limited to prokaryotic transposases. The Hermes transposon from the housefly is part of the eukaryotic hAT superfamily that includes hobo from Drosophila, McClintock's maize Activator and Tam3 from snapdragon. We report here the three-dimensional structure of a functionally active form of the transposase from Hermes at 2.1-A resolution. The Hermes protein has some structural features of prokaryotic transposases, including a domain with a retroviral integrase fold. However, this domain is disrupted by the insertion of an additional domain. Finally, transposition is observed only when Hermes assembles into a hexamer.

PubMed: 16041385
DOI: 10.1038/NSMB970

実験手法

X-RAY DIFFRACTION (2 Å)