2BVU

D83R mutant of Asaris suum major sperm protein (MSP)

2BVU の概要

• エントリーDOI: 10.2210/pdb2bvu/pdb
• 関連するPDBエントリー: 1MSP 3MSP
• 分子名称: MAJOR SPERM PROTEIN, ISOFORM ALPHA (2 entities in total)
• 機能のキーワード: cytosketal protein, major sperm protein, structural protein
• 由来する生物種: ASCARIS SUUM (PIG ROUNDWORM)
• 細胞内の位置: Cell projection, pseudopodium: P27439
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 57280.86

構造登録者

Stewart, M.,Grant, R.P.,Roberts, T.M. (登録日: 2005-07-04, 公開日: 2006-10-25, 最終更新日: 2023-12-13)

主引用文献

Miao, L.,Vanderlinde, O.,Liu, J.,Grant, R.P.,Wouterse, A.,Shimabukuro, K.,Philipse, A.,Stewart, M.,Roberts, T.M.
The Role of Filament-Packing Dynamics in Powering Amoeboid Cell Motility.
Proc.Natl.Acad.Sci.USA, 105:5390-, 2008

PubMed Abstract: Although several models have been proposed to account for how cytoskeleton polymerization drives protrusion in cell motility, the precise mechanism remains controversial. Here, we show that, in addition to force exerted directly against the membrane by growing filaments, the way elongating filaments pack also contributes to protrusion by generating an expansion of the cytoskeleton gel. Tomography shows that filament packing in the major sperm protein (MSP) -based nematode sperm-motility machinery resembles that observed with rigid rods. Maximum rod-packing density decreases dramatically as the rods lengthen. Therefore, as filaments elongate, the cytoskeleton gel expands to accommodate their packing less densely. This volume expansion combines with polymerization to drive protrusion. Consistent with this hypothesis, an engineered MSP mutant that generates shorter filaments shows higher filament-packing density and slower movement.

PubMed: 18385381
DOI: 10.1073/PNAS.0708416105

実験手法

X-RAY DIFFRACTION (2.5 Å)