2BOP

CRYSTAL STRUCTURE AT 1.7 ANGSTROMS OF THE BOVINE PAPILLOMAVIRUS-1 E2 DNA-BINDING DOMAIN BOUND TO ITS DNA TARGET

2BOP の概要

• エントリーDOI: 10.2210/pdb2bop/pdb
• 分子名称: DNA (5'-D(*CP*CP*GP*AP*CP*CP*GP*AP*CP*GP*TP*CP*GP*GP*TP*CP*G )-3'), PROTEIN (E2), YTTERBIUM (III) ION, ... (4 entities in total)
• 機能のキーワード: protein-dna complex, double helix, transcription-dna complex, transcription/dna
• 由来する生物種: Bovine papillomavirus type 1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15156.32

構造登録者

Hegde, R.S.,Grossman, S.R.,Laimins, L.A.,Sigler, P.B. (登録日: 1994-01-13, 公開日: 1994-01-31, 最終更新日: 2024-02-14)

主引用文献

Hegde, R.S.,Grossman, S.R.,Laimins, L.A.,Sigler, P.B.
Crystal structure at 1.7 A of the bovine papillomavirus-1 E2 DNA-binding domain bound to its DNA target.
Nature, 359:505-512, 1992

PubMed Abstract: The dominant transcriptional regulator of the papillomaviruses, E2, binds to its specific DNA target through a previously unobserved dimeric antiparallel beta-barrel. The DNA is severely but smoothly bent over the barrel by the interaction of successive major grooves with a pair of symmetrically disposed alpha-helices. The specific interface is an 'interwoven' network of interactions where the identifying base pairs of the target contact more than one amino-acid side chain and the discriminating amino acids interact with more than one base pair.

PubMed: 1328886
DOI: 10.1038/359505a0

実験手法

X-RAY DIFFRACTION (1.7 Å)