2BOJ
crystal Structure of pseudomonas aeruginosa lectin (PA-IIL) complexed with methyl-B-D-Arabinopyranoside
Summary for 2BOJ
| Entry DOI | 10.2210/pdb2boj/pdb |
| Related | 1GZT 1OUR 1OUS 1OUX 1OVP 1OVS 1OXC 1UZV 1W43 1W8F 1W8H 2BP6 |
| Descriptor | PSEUDOMONAS AERUGINOSA LECTIN II, methyl beta-D-arabinopyranoside, CALCIUM ION, ... (5 entities in total) |
| Functional Keywords | lectin, arabinose, calcium, lewis a, cystic fibrosis |
| Biological source | PSEUDOMONAS AERUGINOSA |
| Total number of polymer chains | 4 |
| Total formula weight | 48012.14 |
| Authors | Sabin, C.D.,Mitchell, E.P.,Wimmerova, M.,Imberty, A. (deposition date: 2005-04-12, release date: 2006-02-22, Last modification date: 2023-12-13) |
| Primary citation | Sabin, C.D.,Mitchell, E.P.,Pokarna, M.,Gautier, C.,Utille, J.-P.,Wimmerova, M.,Imberty, A. Binding of Different Monosaccharides by Lectin Pa-Iil from Pseudoman Aeruginosa: Thermodynamics Data Correlated with X-Ray Structures. FEBS Lett., 580:982-, 2006 Cited by PubMed Abstract: The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host recognition and biofilm formation. Lectin not only displays an unusually high affinity for fucose but also binds to L-fucose, L-galactose and D-arabinose that differ only by the group at position 5 of the sugar ring. Isothermal calorimetry experiments provided precise determination of affinity for the three methyl-glycosides and revealed a large enthalpy contribution. The crystal structures of the complexes of PA-IIL with L-galactose and Met-beta-D-arabinoside have been determined and compared with the PA-IIL/fucose complex described previously. A combination of the structures and thermodynamics provided clues for the role of the hydrophobic group in affinity. PubMed: 16438968DOI: 10.1016/J.FEBSLET.2006.01.030 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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