2BOJ

crystal Structure of pseudomonas aeruginosa lectin (PA-IIL) complexed with methyl-B-D-Arabinopyranoside

2BOJ の概要

• エントリーDOI: 10.2210/pdb2boj/pdb
• 関連するPDBエントリー: 1GZT 1OUR 1OUS 1OUX 1OVP 1OVS 1OXC 1UZV 1W43 1W8F 1W8H 2BP6
• 分子名称: PSEUDOMONAS AERUGINOSA LECTIN II, methyl beta-D-arabinopyranoside, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: lectin, arabinose, calcium, lewis a, cystic fibrosis
• 由来する生物種: PSEUDOMONAS AERUGINOSA
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 48012.14

構造登録者

Sabin, C.D.,Mitchell, E.P.,Wimmerova, M.,Imberty, A. (登録日: 2005-04-12, 公開日: 2006-02-22, 最終更新日: 2023-12-13)

主引用文献

Sabin, C.D.,Mitchell, E.P.,Pokarna, M.,Gautier, C.,Utille, J.-P.,Wimmerova, M.,Imberty, A.
Binding of Different Monosaccharides by Lectin Pa-Iil from Pseudoman Aeruginosa: Thermodynamics Data Correlated with X-Ray Structures.
FEBS Lett., 580:982-, 2006

PubMed Abstract: The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host recognition and biofilm formation. Lectin not only displays an unusually high affinity for fucose but also binds to L-fucose, L-galactose and D-arabinose that differ only by the group at position 5 of the sugar ring. Isothermal calorimetry experiments provided precise determination of affinity for the three methyl-glycosides and revealed a large enthalpy contribution. The crystal structures of the complexes of PA-IIL with L-galactose and Met-beta-D-arabinoside have been determined and compared with the PA-IIL/fucose complex described previously. A combination of the structures and thermodynamics provided clues for the role of the hydrophobic group in affinity.

PubMed: 16438968
DOI: 10.1016/J.FEBSLET.2006.01.030

実験手法

X-RAY DIFFRACTION (1.8 Å)