2BMO

The Crystal Structure of Nitrobenzene Dioxygenase

2BMO の概要

• エントリーDOI: 10.2210/pdb2bmo/pdb
• 関連するPDBエントリー: 2BMQ 2BMR
• 分子名称: OXYGENASE-ALPHA NBDO, OXYGENASE-BETA NBDO, FE2/S2 (INORGANIC) CLUSTER, ... (8 entities in total)
• 機能のキーワード: nitrobenzene dioxygenase, nitroarene, rieske non-heme dioxygenase, substrate specificity iron-sulfur, metal-binding, nad, oxidoreductase
• 由来する生物種: COMAMONAS SP.; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 74135.05

構造登録者

Friemann, R.,Ivkovic-Jensen, M.M.,Lessner, D.J.,Yu, C.,Gibson, D.T.,Parales, R.E.,Eklund, H.,Ramaswamy, S. (登録日: 2005-03-15, 公開日: 2005-05-04, 最終更新日: 2023-12-13)

主引用文献

Friemann, R.,Ivkovic-Jensen, M.M.,Lessner, D.J.,Yu, C.L.,Gibson, D.T.,Parales, R.E.,Eklund, H.,Ramaswamy, S.
Structural insight into the dioxygenation of nitroarene compounds: the crystal structure of nitrobenzene dioxygenase.
J. Mol. Biol., 348:1139-1151, 2005

PubMed Abstract: Nitroaromatic compounds are used extensively in many industrial processes and have been released into the environment where they are considered environmental pollutants. Nitroaromatic compounds, in general, are resistant to oxidative attack due to the electron-withdrawing nature of the nitro groups and the stability of the benzene ring. However, the bacterium Comamonas sp. strain JS765 can grow with nitrobenzene as a sole source of carbon, nitrogen and energy. Biodegradation is initiated by the nitrobenzene dioxygenase (NBDO) system. We have determined the structure of NBDO, which has a hetero-hexameric structure similar to that of several other Rieske non-heme iron dioxygenases. The catalytic subunit contains a Rieske iron-sulfur center and an active-site mononuclear iron atom. The structures of complexes with substrates nitrobenzene and 3-nitrotoluene reveal the structural basis for its activity with nitroarenes. The substrate pocket contains an asparagine residue that forms a hydrogen bond to the nitro-group of the substrate, and orients the substrate in relation to the active-site mononuclear iron atom, positioning the molecule for oxidation at the nitro-substituted carbon.

PubMed: 15854650
DOI: 10.1016/j.jmb.2005.03.052

実験手法

X-RAY DIFFRACTION (1.2 Å)