2BKV

Structure and kinetics of a monomeric glucosamine-6-phosphate deaminase: missing link of the NagB superfamily

2BKV の概要

• エントリーDOI: 10.2210/pdb2bkv/pdb
• 関連するPDBエントリー: 2BKX
• 分子名称: GLUCOSAMINE-6-PHOSPHATE DEAMINASE, 2-PHOSPHOGLYCOLIC ACID (3 entities in total)
• 機能のキーワード: hydrolase, substrate inhibition, fructose-6-phosphate
• 由来する生物種: BACILLUS SUBTILIS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54362.75

構造登録者

Vincent, F.,Davies, G.J.,Brannigan, J.A. (登録日: 2005-02-21, 公開日: 2005-03-09, 最終更新日: 2024-05-01)

主引用文献

Vincent, F.,Davies, G.J.,Brannigan, J.A.
Structure and Kinetics of a Monomeric Glucosamine 6-Phosphate Deaminase: Missing Link of the Nagb Superfamily
J.Biol.Chem., 280:19649-, 2005

PubMed Abstract: Glucosamine 6-phosphate is converted to fructose 6-phosphate and ammonia by the action of the enzyme glucosamine 6-phosphate deaminase, NagB. This reaction is the final step in the specific GlcNAc utilization pathway and thus decides the metabolic fate of GlcNAc. Sequence analyses suggest that the NagB "superfamily" consists of three main clusters: multimeric and allosterically regulated glucosamine-6-phosphate deaminases (exemplified by Escherichia coli NagB), phosphogluconolactonases, and monomeric hexosamine-6-phosphate deaminases. Here we present the three-dimensional structure and kinetics of the first member of this latter group, the glucosamine-6-phosphate deaminase, NagB, from Bacillus subtilis. The structures were determined in ligand-complexed forms at resolutions around 1.4 Angstroms. BsuNagB is monomeric in solution and as a consequence is active (k(cat) 28 s(-1), K(m(app)) 0.13 mM) without the need for allosteric activators. A decrease in activity at high substrate concentrations may reflect substrate inhibition (with K(i) of approximately 4 mM). The structure completes the NagB superfamily structural landscape and thus allows further interrogation of genomic data in terms of the regulation of NagB and the metabolic fate(s) of glucosamine 6-phosphate.

PubMed: 15755726
DOI: 10.1074/JBC.M502131200

実験手法

X-RAY DIFFRACTION (1.5 Å)