2BK5

Human Monoamine Oxidase B: I199F mutant in complex with isatin

2BK5 の概要

• エントリーDOI: 10.2210/pdb2bk5/pdb
• 関連するPDBエントリー: 1GOS 1H8R 1OJ9 1OJA 1OJB 1OJC 1OJD 1S2Q 1S2Y 1S3B 1S3E 2BK3 2BK4
• 分子名称: AMINE OXIDASE [FLAVIN-CONTAINING] B, FLAVIN-ADENINE DINUCLEOTIDE, ISATIN, ... (4 entities in total)
• 機能のキーワード: oxidoreductase, fad-containing amine oxidase, maob acetylation, fad, flavoprotein, maob, transmembrane
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side: P27338
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 119608.85

構造登録者

Binda, C.,Edmondson, D.E.,Mattevi, A.,Hubalek, F.,Khalil, A.,Li, M.,Castagnoli, N. (登録日: 2005-02-10, 公開日: 2005-02-14, 最終更新日: 2024-10-09)

主引用文献

Hubalek, F.,Binda, C.,Khalil, A.,Li, M.,Mattevi, A.,Castagnoli, N.,Edmondson, D.E.
Demonstration of Isoleucine 199 as a Structural Determinant for the Selective Inhibition of Human Monoamine Oxidase B by Specific Reversible Inhibitors.
J.Biol.Chem., 280:15761-, 2005

PubMed Abstract: Several reversible inhibitors selective for human monoamine oxidase B (MAO B) that do not inhibit MAO A have been described in the literature. The following compounds: 8-(3-chlorostyryl)caffeine, 1,4-diphenyl-2-butene, and trans,trans-farnesol are shown to inhibit competitively human, horse, rat, and mouse MAO B with K(i) values in the low micromolar range but are without effect on either bovine or sheep MAO B or human MAO A. In contrast, the reversible competitive inhibitor isatin binds to all known MAO B and MAO A with similar affinities. Sequence alignments and the crystal structures of human MAO B in complex with 1,4-diphenyl-2-butene or with trans,trans-farnesol provide molecular insights into these specificities. These inhibitors span the substrate and entrance cavities with the side chain of Ile-199 rotated out of its normal conformation suggesting that Ile-199 is gating the substrate cavity. Ile-199 is conserved in all known MAO B sequences except bovine MAO B, which has Phe in this position (the sequence of sheep MAO B is unknown). Phe is conserved in the analogous position in MAO A sequences. The human MAO B I199F mutant protein of MAO B binds to isatin (K(i) = 3 microM) but not to the three inhibitors listed above. The crystal structure of this mutant demonstrates that the side chain of Phe-199 interferes with the binding of those compounds. This suggests that the Ile-199 "gate" is a determinant for the specificity of these MAO B inhibitors and provides a molecular basis for the development of MAO B-specific reversible inhibitors without interference with MAO A function in neurotransmitter metabolism.

PubMed: 15710600
DOI: 10.1074/JBC.M500949200

実験手法

X-RAY DIFFRACTION (1.83 Å)