2BJO

Crystal Structure of the Organic Hydroperoxide Resistance Protein OhrB of Bacillus subtilis

Summary for 2BJO

• Entry DOI: 10.2210/pdb2bjo/pdb
• Descriptor: ORGANIC HYDROPEROXIDE RESISTANCE PROTEIN OHRB (2 entities in total)
• Functional Keywords: organic hydroperoxide resistance, heat shock protein, oxidoreductase
• Biological source: BACILLUS SUBTILIS
• Total number of polymer chains: 2
• Total formula weight: 28888.31

Authors

Cooper, D.R.,Surendranath, Y.,Bielnicki, J.,Devedjiev, Y.,Joachimiak, A.,Derewenda, Z.S. (deposition date: 2005-02-04, release date: 2006-03-09, Last modification date: 2024-11-13)

Primary citation

Cooper, D.R.,Surendranath, Y.,Devedjiev, Y.,Bielnicki, J.,Derewenda, Z.S.
Structure of the Bacillus Subtilis Ohrb Hydroperoxide-Resistance Protein in a Fully Oxidized State.
Acta Crystallogr.,Sect.D, 63:1269-, 2007

PubMed Abstract: The crystal structure of the fully oxidized form of the Bacillus subtilis organic hydroperoxide-resistance (OhrB) protein is reported at 2.1 A resolution. The electron density reveals an intact catalytic disulfide bond (Cys55-Cys119) in each of the two molecules, which are intertwined into a canonical obligate dimer. However, the stereochemistry of the disulfides is unorthodox and strained, suggesting that they are sensitive to reducing agents. A deep solvent-accessible gorge reaching Cys55 may represent the access route for the reductant.

PubMed: 18084074
DOI: 10.1107/S0907444907050226

Experimental method

X-RAY DIFFRACTION (2.1 Å)