2BHM

Crystal structure of VirB8 from Brucella suis

2BHM の概要

• エントリーDOI: 10.2210/pdb2bhm/pdb
• 分子名称: TYPE IV SECRETION SYSTEM PROTEIN VIRB8 (2 entities in total)
• 機能のキーワード: bacterial protein, bacterial type iv secretion
• 由来する生物種: BRUCELLA MELITENSIS BIOVAR SUIS (BRUCELLA SUIS)
• 細胞内の位置: Cell inner membrane; Single-pass membrane protein (Potential): Q7CEG3
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 91100.73

構造登録者

Bayliss, R.,Baron, C.,Waksman, G. (登録日: 2005-01-14, 公開日: 2005-03-16, 最終更新日: 2024-05-08)

主引用文献

Terradot, L.,Bayliss, R.,Oomen, C.,Leonard, G.,Baron, C.,Waksman, G.
Structures of Two Core Subunits of the Bacterial Type Iv Secretion System, Virb8 from Brucella Suis and Comb10 from Helicobacter Pylori
Proc.Natl.Acad.Sci.USA, 102:4596-, 2005

PubMed Abstract: Type IV secretion systems (T4SSs) are commonly used secretion machineries in Gram-negative bacteria. They are used in the infection of human, animal, or plant cells and the propagation of antibiotic resistance. The T4SS apparatus spans both membranes of the bacterium and generally is composed of 12 proteins, named VirB1-11 and VirD4 after proteins of the canonical Agrobacterium tumefaciens T4SS. The periplasmic core complex of VirB8/VirB10 structurally and functionally links the cytoplasmic NTPases of the system with its outer membrane and pilus components. Here we present crystal structures of VirB8 of Brucella suis, the causative agent of brucellosis, and ComB10, a VirB10 homolog of Helicobacter pylori, the causative agent of gastric ulcers. The structures of VirB8 and ComB10 resemble known folds, albeit with novel secondary-structure modifications unique to and conserved within their respective families. Both proteins crystallized as dimers, providing detailed predictions about their self associations. These structures make a substantial contribution to the repertoire of T4SS component structures and will serve as springboards for future functional and protein-protein interaction studies by using knowledge-based site-directed and deletion mutagenesis.

PubMed: 15764702
DOI: 10.1073/PNAS.0408927102

実験手法

X-RAY DIFFRACTION (2.4 Å)