2BGW

XPF from Aeropyrum pernix, complex with DNA

2BGW の概要

• エントリーDOI: 10.2210/pdb2bgw/pdb
• 分子名称: XPF ENDONUCLEASE, 5'-D(*GP*AP*TP*CP*AP*CP*AP*GP*AP*TP *GP*CP*TP*GP*A)-3', 5'-D(*TP*CP*AP*GP*CP*AP*TP*CP*TP*GP *TP*GP*AP*TP*C)-3', ... (6 entities in total)
• 機能のキーワード: hydrolase, structure specific endonuclease, nucleotide excision repair
• 由来する生物種: Aeropyrum pernix; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 59792.55

構造登録者

Newman, M.,Murray-Rust, J.,Lally, J.,Rudolf, J.,Fadden, A.,Knowles, P.P.,White, M.F.,McDonald, N.Q. (登録日: 2005-01-06, 公開日: 2005-02-23, 最終更新日: 2023-12-13)

主引用文献

Newman, M.,Murray-Rust, J.,Lally, J.,Rudolf, J.,Fadden, A.,Knowles, P.P.,White, M.F.,McDonald, N.Q.
Structure of an XPF endonuclease with and without DNA suggests a model for substrate recognition.
EMBO J., 24:895-905, 2005

PubMed Abstract: The XPF/Mus81 structure-specific endonucleases cleave double-stranded DNA (dsDNA) within asymmetric branched DNA substrates and play an essential role in nucleotide excision repair, recombination and genome integrity. We report the structure of an archaeal XPF homodimer alone and bound to dsDNA. Superposition of these structures reveals a large domain movement upon binding DNA, indicating how the (HhH)(2) domain and the nuclease domain are coupled to allow the recognition of double-stranded/single-stranded DNA junctions. We identify two nonequivalent DNA-binding sites and propose a model in which XPF distorts the 3' flap substrate in order to engage both binding sites and promote strand cleavage. The model rationalises published biochemical data and implies a novel role for the ERCC1 subunit of eukaryotic XPF complexes.

PubMed: 15719018
DOI: 10.1038/sj.emboj.7600581

実験手法

X-RAY DIFFRACTION (2.8 Å)