2BFU

X-ray structure of CPMV top component

2BFU の概要

• エントリーDOI: 10.2210/pdb2bfu/pdb
• 関連するPDBエントリー: 1BMV 1NY7
• 分子名称: COWPEA MOSAIC VIRUS, LARGE (L) SUBUNIT, COWPEA MOSAIC VIRUS, SMALL (S) SUBUNIT (2 entities in total)
• 機能のキーワード: comovirus, virus, viral coat protein, cowpea mosaic virus cpmv, top component, nanotechnology, empty particles, icosahedral virus
• 由来する生物種: COWPEA MOSAIC VIRUS; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 61820.00

構造登録者

Ochoa, W.F.,Chatterji, A.,Lin, T.,Johnson, J.E. (登録日: 2004-12-13, 公開日: 2005-01-12, 最終更新日: 2024-10-16)

主引用文献

Ochoa, W.F.,Chatterji, A.,Lin, T.,Johnson, J.E.
Generation and Structural Analysis of Reactive Empty Particles Derived from an Icosahedral Virus.
Chem.Biol., 13:771-, 2006

PubMed Abstract: Chemical and genetic modifications on the surface of viral protein cages confer unique properties to the virus particles with potential nano and biotechnological applications. The enclosed space in the interior of the virus particles further increases its versatility as a nanomaterial. In this paper, we report a simple method to generate a high yield of stable cowpea mosaic virus (CPMV) empty capsids from their native nucleoprotein counterparts by removing the encapsidated viral genome without compromising the integrity of the protein coat. Biochemical and structural comparison of artificially generated empty particles did not reveal any distinguishable differences from CPMV particles containing viral RNA. Preliminary results on the use of artificially produced empty CPMV capsids as a carrier capsule are described.

PubMed: 16873025
DOI: 10.1016/J.CHEMBIOL.2006.05.014

実験手法

X-RAY DIFFRACTION (4 Å)