2BFN

The crystal structure of the complex of the haloalkane dehalogenase LinB with the product of dehalogenation reaction 1,2-dichloropropane.

2BFN の概要

• エントリーDOI: 10.2210/pdb2bfn/pdb
• 関連するPDBエントリー: 1CV2 1D07 1G42 1G4H 1G5F 1IZ7 1IZ8 1K5P 1K63 1K6E 1MJ5
• 分子名称: HALOGENALKANE DEHALOGENASE, (2S)-2,3-DICHLOROPROPAN-1-OL, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: haloalkane dehalogenase linb, 1\, 2\, 3-trichloropropane, hydrolase, alpha/beta-hydrolase
• 由来する生物種: SPHINGOMONAS PAUCIMOBILIS
• 細胞内の位置: Periplasm: P51698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33429.28

構造登録者

Banas, P.,Otyepka, M.,Jerabek, P.,Vevodova, J.,Bohac, M.,Damborsky, J. (登録日: 2004-12-09, 公開日: 2006-06-26, 最終更新日: 2023-12-13)

主引用文献

Minincova, M.,Prokop, Z.,Vevodova, J.,Nagata, Y.,Damborsky, J.
Weak Activity of Haloalkane Dehalogenase Linb with 1,2,3-Trichloropropane Revealed by X-Ray Crystallography and Microcalorimetry
Appl.Environ.Microbiol., 73:2005-, 2007

PubMed Abstract: 1,2,3-Trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes that catalyze the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been considered inactive with TCP, since the reaction cannot be easily detected by conventional analytical methods. Here we demonstrate detection of the weak activity (k(cat) = 0.005 s(-1)) of LinB with TCP using X-ray crystallography and microcalorimetry. This observation makes LinB a useful starting material for the development of a new biocatalyst toward TCP by protein engineering. Microcalorimetry is proposed to be a universal method for the detection of weak enzymatic activities. Detection of these activities is becoming increasingly important for engineering novel biocatalysts using the scaffolds of proteins with promiscuous activities.

PubMed: 17259360
DOI: 10.1128/AEM.02416-06

実験手法

X-RAY DIFFRACTION (1.6 Å)