2BF1

Structure of an unliganded and fully-glycosylated SIV gp120 envelope glycoprotein

Summary for 2BF1

• Entry DOI: 10.2210/pdb2bf1/pdb
• Descriptor: EXTERIOR MEMBRANE GLYCOPROTEIN GP120, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• Functional Keywords: virus protein, siv, gp120, envelope glycoprotein, aids, coat protein
• Biological source: SIMIAN IMMUNODEFICIENCY VIRUS (SIV)
• Total number of polymer chains: 1
• Total formula weight: 46048.21

Authors

Chen, B.,Vogan, E.M.,Gong, H.,Skehel, J.J.,Wiley, D.C.,Harrison, S.C. (deposition date: 2004-12-02, release date: 2005-02-17, Last modification date: 2024-10-16)

Primary citation

Chen, B.,Vogan, E.M.,Gong, H.,Skehel, J.J.,Wiley, D.C.,Harrison, S.C.
Structure of an Unliganded Simian Immunodeficiency Virus Gp120 Core
Nature, 433:834-, 2005

PubMed Abstract: Envelope glycoproteins of human and simian immunodeficiency virus (HIV and SIV) undergo a series of conformational changes when they interact with receptor (CD4) and co-receptor on the surface of a potential host cell, leading ultimately to fusion of viral and cellular membranes. Structures of fragments of gp120 and gp41 from the envelope protein are known, in conformations corresponding to their post-attachment and postfusion states, respectively. We report the crystal structure, at 4 A resolution, of a fully glycosylated SIV gp120 core, in a conformation representing its prefusion state, before interaction with CD4. Parts of the protein have a markedly different organization than they do in the CD4-bound state. Comparison of the unliganded and CD4-bound structures leads to a model for events that accompany receptor engagement of an envelope glycoprotein trimer. The two conformations of gp120 also present distinct antigenic surfaces. We identify the binding site for a compound that inhibits viral entry.

PubMed: 15729334
DOI: 10.1038/NATURE03327

Experimental method

X-RAY DIFFRACTION (4 Å)