2BCX

Crystal structure of calmodulin in complex with a ryanodine receptor peptide

2BCX の概要

• エントリーDOI: 10.2210/pdb2bcx/pdb
• 分子名称: Calmodulin, Ryanodine receptor 1, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: ef-hand, type-2 turn, calcium binding protein
• 由来する生物種: Gallus gallus (chicken); 詳細
• 細胞内の位置: Sarcoplasmic reticulum membrane ; Multi-pass membrane protein : P11716
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20508.10

構造登録者

Maximciuc, A.A.,Shamoo, Y.,MacKenzie, K.R. (登録日: 2005-10-19, 公開日: 2006-10-31, 最終更新日: 2024-02-14)

主引用文献

Maximciuc, A.A.,Putkey, J.A.,Shamoo, Y.,Mackenzie, K.R.
Complex of calmodulin with a ryanodine receptor target reveals a novel, flexible binding mode.
Structure, 14:1547-1556, 2006

PubMed Abstract: Calmodulin regulates ryanodine receptor-mediated Ca(2+) release through a conserved binding site. The crystal structure of Ca(2+)-calmodulin bound to this conserved site reveals that calmodulin recognizes two hydrophobic anchor residues at a novel "1-17" spacing that brings the calmodulin lobes close together but prevents them from contacting one another. NMR residual dipolar couplings demonstrate that the detailed structure of each lobe is preserved in solution but also show that the lobes experience domain motions within the complex. FRET measurements confirm the close approach of the lobes in binding the 1-17 target and show that calmodulin binds with one lobe to a peptide lacking the second anchor. We suggest that calmodulin regulates the Ca(2+) channel by switching between the contiguous binding mode seen in our crystal structure and a state where one lobe of calmodulin contacts the conserved binding site while the other interacts with a noncontiguous site on the channel.

PubMed: 17027503
DOI: 10.1016/j.str.2006.08.011

実験手法

X-RAY DIFFRACTION (2 Å)