2BC3

T7-tagged full-length streptavidin

2BC3 の概要

• エントリーDOI: 10.2210/pdb2bc3/pdb
• 分子名称: Streptavidin, GLYCEROL, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: streptavidin, t7 tag, biotin binding protein
• 由来する生物種: Streptomyces avidinii
• 細胞内の位置: Secreted: P22629
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33420.29

構造登録者

Stenkamp, R.E.,Le Trong, I.,Ward, T.R.,Humbert, N. (登録日: 2005-10-18, 公開日: 2005-10-25, 最終更新日: 2024-04-03)

主引用文献

Le Trong, I.,Humbert, N.,Ward, T.R.,Stenkamp, R.E.
Crystallographic Analysis of a Full-length Streptavidin with Its C-terminal Polypeptide Bound in the Biotin Binding Site.
J.Mol.Biol., 356:738-745, 2006

PubMed Abstract: The structure of a full-length streptavidin has been determined at 1.7 A resolution and shows that the 20 residue extension at the C terminus forms a well-ordered polypeptide loop on the surface of the tetramer. Residues 150-153 of the extension are bound to the ligand-binding site, possibly competing with exogenous ligands. The binding mode of these residues is compared with that of biotin and peptidic ligands. The observed structure helps to rationalize the observations that full-length mature streptavidin binds biotinylated macromolecules with reduced affinity.

PubMed: 16384581
DOI: 10.1016/j.jmb.2005.11.086

実験手法

X-RAY DIFFRACTION (1.54 Å)