2BAM

RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA AND CALCIUM IONS (PRE-REACTIVE COMPLEX).

2BAM の概要

• エントリーDOI: 10.2210/pdb2bam/pdb
• 分子名称: DNA (5'-D(*TP*AP*TP*GP*GP*AP*TP*CP*CP*AP*TP*A)-3'), PROTEIN (ENDONUCLEASE BAMHI), CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: phosphodiesterase, complex (endonuclease-dna), nuclease, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Bacillus amyloliquefaciens
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 56607.58

構造登録者

Viadiu, H.,Aggarwal, A.K. (登録日: 1998-08-19, 公開日: 1999-10-31, 最終更新日: 2023-08-23)

主引用文献

Viadiu, H.,Aggarwal, A.K.
The role of metals in catalysis by the restriction endonuclease BamHI.
Nat.Struct.Biol., 5:910-916, 1998

PubMed Abstract: Type II restriction enzymes are characterized by their remarkable specificity and simplicity. They require only divalent metals (such as Mg2+ or Mn2+) as cofactors to catalyze the hydrolysis of DNA. However, most of the structural work on endonucleases has been performed in the absence of metals, leaving unanswered questions about their mechanisms of DNA cleavage. Here we report structures of the endonuclease BamHI-DNA complex, determined in the presence of Mn2+ and Ca2+, that describe the enzyme at different stages of catalysis. Overall, the results support a two-metal mechanism of DNA cleavage for BamHI which is distinct from that of EcoRV.

PubMed: 9783752
DOI: 10.1038/2352

実験手法

X-RAY DIFFRACTION (2 Å)