2B9R

Crystal Structure of Human Cyclin B1

2B9R の概要

• エントリーDOI: 10.2210/pdb2b9r/pdb
• 分子名称: Human cyclin B1 (2 entities in total)
• 機能のキーワード: cyclin, cell cycle
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P14635
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 61680.28

構造登録者

Basavappa, R.,Petri, E. (登録日: 2005-10-12, 公開日: 2006-10-17, 最終更新日: 2023-08-23)

主引用文献

Petri, E.T.,Errico, A.,Escobedo, L.,Hunt, T.,Basavappa, R.
The Crystal Structure of Human Cyclin B.
Cell Cycle, 6:1342-1349, 2007

PubMed Abstract: Cyclin B is the key regulatory protein controlling mitosis in all eukaryotes, where it binds cyclin-dependent kinase, cdk1, forming a complex which initiates the mitotic program through phosphorylation of select proteins. Cyclin B regulates the activation, subcellular localization, and substrate specificity of cdk1, and destruction of cyclin B is necessary for mitotic exit. Overexpression of human cyclin B1 has been found in numerous cancers and has been associated with tumor aggressiveness. Here we report the crystal structure of human cyclin B1 to 2.9 A. Comparison of the structure with cyclin A and cyclin E reveals remarkably similar N-terminal cyclin box motifs but significant differences among the C-terminal cyclin box lobes. Divergence in sequence gives rise to unique interaction surfaces at the proposed cyclin B/cdk1 interface as well as the 'RxL' motif substrate binding site on cyclin B. Examination of the structure provides insight into the molecular basis for differential affinities of protein based cyclin/cdk inhibitors such as p27, substrate recognition, and cdk interaction.

PubMed: 17495533

実験手法

X-RAY DIFFRACTION (2.9 Å)