2B7M
Crystal Structure of the S. cerevisiae Exocyst Component Exo70p
Summary for 2B7M
| Entry DOI | 10.2210/pdb2b7m/pdb |
| Descriptor | Exocyst complex component EXO70 (1 entity in total) |
| Functional Keywords | exocyst, exocytosis, endocytosis-exocytosis complex, endocytosis/exocytosis |
| Biological source | Saccharomyces cerevisiae (baker's yeast) |
| Cellular location | Bud: P19658 |
| Total number of polymer chains | 4 |
| Total formula weight | 262149.62 |
| Authors | Hamburger, Z.A.,Hamburger, A.E.,West, A.P.,Weis, W.I. (deposition date: 2005-10-04, release date: 2005-11-15, Last modification date: 2011-07-13) |
| Primary citation | Hamburger, Z.A.,Hamburger, A.E.,West, A.P.,Weis, W.I. Crystal Structure of the S.cerevisiae Exocyst Component Exo70p J.Mol.Biol., 356:9-21, 2006 Cited by PubMed Abstract: The exocyst is an evolutionarily conserved multiprotein complex required for the targeting and docking of post-Golgi vesicles to the plasma membrane. Through its interactions with a variety of proteins, including small GTPases, the exocyst is thought to integrate signals from the cell and signal that vesicles arriving at the plasma membrane are ready for fusion. Here we describe the three-dimensional crystal structure of one of the components of the exocyst, Exo70p, from Saccharomyces cerevisiae at 3.5A resolution. Exo70p binds the small GTPase Rho3p in a GTP-dependent manner with an equilibrium dissociation constant of approximately 70 microM. Exo70p is an extended rod approximately 155 angstroms in length composed principally of alpha helices, and is a novel fold. The structure provides a first view of the Exo70 protein family and provides a framework to study the molecular function of this exocyst component. PubMed: 16359701DOI: 10.1016/j.jmb.2005.09.099 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.5 Å) |
Structure validation
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