2B7M

Crystal Structure of the S. cerevisiae Exocyst Component Exo70p

2B7M の概要

• エントリーDOI: 10.2210/pdb2b7m/pdb
• 分子名称: Exocyst complex component EXO70 (1 entity in total)
• 機能のキーワード: exocyst, exocytosis, endocytosis-exocytosis complex, endocytosis/exocytosis
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Bud: P19658
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 262149.62

構造登録者

Hamburger, Z.A.,Hamburger, A.E.,West, A.P.,Weis, W.I. (登録日: 2005-10-04, 公開日: 2005-11-15, 最終更新日: 2011-07-13)

主引用文献

Hamburger, Z.A.,Hamburger, A.E.,West, A.P.,Weis, W.I.
Crystal Structure of the S.cerevisiae Exocyst Component Exo70p
J.Mol.Biol., 356:9-21, 2006

PubMed Abstract: The exocyst is an evolutionarily conserved multiprotein complex required for the targeting and docking of post-Golgi vesicles to the plasma membrane. Through its interactions with a variety of proteins, including small GTPases, the exocyst is thought to integrate signals from the cell and signal that vesicles arriving at the plasma membrane are ready for fusion. Here we describe the three-dimensional crystal structure of one of the components of the exocyst, Exo70p, from Saccharomyces cerevisiae at 3.5A resolution. Exo70p binds the small GTPase Rho3p in a GTP-dependent manner with an equilibrium dissociation constant of approximately 70 microM. Exo70p is an extended rod approximately 155 angstroms in length composed principally of alpha helices, and is a novel fold. The structure provides a first view of the Exo70 protein family and provides a framework to study the molecular function of this exocyst component.

PubMed: 16359701
DOI: 10.1016/j.jmb.2005.09.099

実験手法

X-RAY DIFFRACTION (3.5 Å)