2B6X

T4 Lysozyme mutant L99A at 200 MPa

2B6X の概要

• エントリーDOI: 10.2210/pdb2b6x/pdb
• 関連するPDBエントリー: 2B6T 2B6W 2B6Y 2B6Z 2B70 2B72 2B73 2B74 2B75
• 分子名称: Lysozyme, CHLORIDE ION, BETA-MERCAPTOETHANOL, ... (4 entities in total)
• 機能のキーワード: high pressure, t4 lysozyme, hydrolase
• 由来する生物種: Enterobacteria phage T4
• 細胞内の位置: Host cytoplasm : P00720
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18586.19

構造登録者

Collins, M.D.,Quillin, M.L.,Matthews, B.W.,Gruner, S.M. (登録日: 2005-10-03, 公開日: 2005-11-08, 最終更新日: 2024-02-14)

主引用文献

Collins, M.D.,Hummer, G.,Quillin, M.L.,Matthews, B.W.,Gruner, S.M.
Cooperative water filling of a nonpolar protein cavity observed by high-pressure crystallography and simulation
Proc.Natl.Acad.Sci.Usa, 102:16668-16671, 2005

PubMed Abstract: Formation of a water-expelling nonpolar core is the paradigm of protein folding and stability. Although experiment largely confirms this picture, water buried in "hydrophobic" cavities is required for the function of some proteins. Hydration of the protein core has also been suggested as the mechanism of pressure-induced unfolding. We therefore are led to ask whether even the most nonpolar protein core is truly hydrophobic (i.e., water-repelling). To answer this question we probed the hydration of an approximately 160-A(3), highly hydrophobic cavity created by mutation in T4 lysozyme by using high-pressure crystallography and molecular dynamics simulation. We show that application of modest pressure causes approximately four water molecules to enter the cavity while the protein itself remains essentially unchanged. The highly cooperative filling is primarily due to a small change in bulk water activity, which implies that changing solvent conditions or, equivalently, cavity polarity can dramatically affect interior hydration of proteins and thereby influence both protein activity and folding.

PubMed: 16269539
DOI: 10.1073/pnas.0508224102

実験手法

X-RAY DIFFRACTION (2.107 Å)